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It has been known that gut absorption also plays an important role in the mechanism underlying hepatic steatosis. Conventional in vitro cell culture models have limitations in recapitulating the mechanisms of hepatic steatosis because it does not include the gut absorption process. Previously, we reported development of a microfluidic gut\u2013liver chip that can recapitulate the gut absorption of fatty acids and subsequent lipid accumulation in liver cells. In this study, we performed a series of experiments to verify that our gut\u2013liver chip reproduces various aspects of hepatic steatosis. The absorption of fatty acids was evaluated under various culture conditions. The anti\u2010steatotic effect of turofexorate isopropyl (XL\u2010335) and metformin was tested, and both drugs showed different action mechanisms. In addition, the oxidative stress induced by lipid absorption was evaluated. Our results demonstrate the potential of the gut\u2013liver chip for use as a novel, physiologically realistic in vitro model to study fatty liver disease.<\/jats:p>","DOI":"10.1002\/btpr.3121","type":"journal-article","created":{"date-parts":[[2021,1,3]],"date-time":"2021-01-03T23:00:01Z","timestamp":1609714801000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["In vitro hepatic steatosis model based on gut\u2013liver\u2010on\u2010a\u2010chip"],"prefix":"10.1002","volume":"37","author":[{"given":"Joong\u2010won","family":"Jeon","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Hongik University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Hwan","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Bionano Engineering Hanyang University  Ansan Republic of 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