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Vaccination is a viable approach to fight against\n                    <jats:italic>C. difficile<\/jats:italic>\n                    and several efforts in this direction are ongoing. Plants are promising vaccine biofactories offering low cost, enhanced safety, and allow for the formulation of oral vaccines. Herein, the CdeM protein, which is a spore antigen associated with immunoprotection against\n                    <jats:italic>C. difficile<\/jats:italic>\n                    , was selected to begin the development of plant\u2010based vaccine candidates. The vaccine antigen is based in a fusion protein (LTB\u2010CdeM), carrying the CdeM antigen, fused to the carboxi\u2010terminus of the B subunit of the\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    heat\u2010labile enterotoxin (LTB) as a mucosal immunogenic carrier. LTB\u2010CdeM was produced in plants using a synthetic optimized gene according codon usage and mRNA stability criteria. The obtained transformed tobacco lines produced the LTB\u2010CdeM antigen in the range of 52\u201390\u2009\u03bcg\/g dry weight leaf tissues. The antigenicity of the plant\u2010made LTB\u2010CdeM antigen was evidenced by GM1\u2010ELISA and immunogenicity assessment performed in test mice revealed that the LTB\u2010CdeM antigen is orally immunogenic inducing humoral responses against CdeM epitopes. This report constitutes the first step in the development of plant\u2010based vaccines against\n                    <jats:italic>C. difficile<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.1002\/btpr.3141","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T07:40:12Z","timestamp":1614930012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Transgenic plants expressing a\n                    <scp>\n                      <i>Clostridium difficile<\/i>\n                    <\/scp>\n                    spore antigen as an approach to develop low\u2010cost oral vaccines"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2569-7329","authenticated-orcid":false,"given":"Sergio","family":"Rosales\u2010Mendoza","sequence":"first","affiliation":[{"name":"Laboratorio de Biofarmac\u00e9uticos Recombinantes Facultad de 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