{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:00:37Z","timestamp":1772118037245,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,8,28]],"date-time":"2021-08-28T00:00:00Z","timestamp":1630108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004748","name":"Mashhad University of Medical Sciences","doi-asserted-by":"publisher","award":["911191"],"award-info":[{"award-number":["911191"]}],"id":[{"id":"10.13039\/501100004748","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The positively\u2010charged peptide antp derived from Antennapedia transcription protein is demonstrated to mediate the liposome translocation across the cell membrane. In the current investigation, we prepared a stable liposomal doxorubicin (Dox) formulation and targeted it with the antp peptide from 0 to 200 ligand\/liposome. These antp\u2010containing liposomes were investigated in terms of physical stability on storage in the refrigerator and upon incubation in blood. Also, other features like cell binding, uptake, biodistribution, and treatment efficiency were evaluated in C26 colon carcinoma BALB\/c mice. The Antp in liposomes resulted in enhanced particle growth with the development of the enormously large liposomes from 2000 to 6000\u2009nm. Upon incubation in blood, these large liposomes were removed. The antp also enhanced the cell binding affinity and cell uptake rate of the liposomes and resulted in the restriction of the cancer cell proliferation, but it failed to improve the chemotherapeutic property of the Dox\u2010liposome. The i.v. injection of antp\u2010liposomes (15\u2009mg Dox\/kg) caused severe body weight loss and early death incidence due to probably increased toxicity. The antp targeting offered no advantage to the Dox\u2010liposome in the delivery of Dox to the tumor, and failed to enhance the treatment efficiency of the liposomes.<\/jats:p>","DOI":"10.1002\/btpr.3202","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T03:13:14Z","timestamp":1629256394000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antennapedia\u2010derived positively\u2010charged peptide faces multiple problems upon their usage as targeting ligand for liposomal doxorubicin"],"prefix":"10.1002","volume":"37","author":[{"given":"Sima","family":"Kousani","sequence":"first","affiliation":[{"name":"Nanotechnology Research Center, Pharmaceutical Technology Institute Mashhad University of Medical Sciences  Mashhad 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