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This work describes fractionation by wet\u2010milling of transgenic maize expressing the B subunit of the heat\u2010labile enterotoxin of <jats:italic>Escherichia coli<\/jats:italic> (LT\u2010B), a potent immunogen and candidate for oral vaccine and vaccine components. The LT\u2010B gene was directed to express in seed by an endosperm specific promoter. Two steeping treatments, traditional steeping (TS, 0.2% SO<jats:sub>2<\/jats:sub> + 0.5% lactic acid) and water steeping (WS, water only), were evaluated to determine effects on recovery of functional LT\u2010B in wet\u2010milled fractions. The overall recovery of the LT\u2010B protein from WS treatment was 1.5\u2010fold greater than that from TS treatment. In both steeping types, LT\u2010B was distributed similarly among the fractions, resulting in enrichment of functional LT\u2010B in fine fiber, coarse fiber and pericarp fractions by concentration factors of 1.5 to 8 relative to the whole kernels on a per\u2010mass basis. Combined with endosperm\u2010specific expression and secretory pathway targeting, wet\u2010milling enables enrichment of high\u2010value recombinant proteins in low\u2010value fractions, such as the fine fiber, and co\u2010utilization of remaining fractions in alternative industrial applications. \u00a9 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010<\/jats:p>","DOI":"10.1002\/btpr.326","type":"journal-article","created":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T11:45:15Z","timestamp":1259063115000},"page":"458-465","source":"Crossref","is-referenced-by-count":8,"title":["Wet\u2010milling transgenic maize seed for fraction enrichment of recombinant subunit vaccine"],"prefix":"10.1002","volume":"26","author":[{"given":"Lorena","family":"Moeller","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raye","family":"Taylor\u2010Vokes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Fox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinglei","family":"Gan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,11,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hch121"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-004-0767-1"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2006.00216.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-006-0449-8"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2008.02.002"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4729-7_11"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4729-7_11"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","unstructured":"Foreign Agricultural Service\/USDA Office of Global Analysis. 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