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Negatively charged latex nanoparticles (NPs) with nominal diameters of 20, 40, and 100\u2009nm are also studied as surrogates for bioparticles. Protein binding and its trends with respect to salt concentration depend on the protein size and are different for the two agarose\u2010based multimodal resins. For the smaller proteins, the amount of protein bound over practical time scales is limited by the resin surface area and is larger for Capto Core 400 compared with Capto Core 700. For the larger proteins, diffusion is severely restricted in Capto Core 400, resulting in lower binding capacities than those observed for Capto Core 700 despite the larger surface area. Adding 500\u2009mM NaCl reduces the local bound protein concentration and diffusional hindrance resulting in higher binding capacities for the large proteins in Capto Core 400 compared with low ionic strength conditions. The NPs are essentially completely excluded from the Capto Core 400 pores. However, 20 and 40\u2009nm NPs bind significantly to Capto Core 700, further hindering protein diffusion. A model is provided to predict the dynamic binding capacities as a function of residence time.<\/jats:p>","DOI":"10.1002\/btpr.3300","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:24:39Z","timestamp":1663115079000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Protein adsorption on core\u2010shell resins for flow\u2010through purifications: Effect of protein molecular size, shape, and salt concentration"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8771-1547","authenticated-orcid":false,"given":"Xue","family":"Mi","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng\u2010Ching","family":"Wang","sequence":"additional","affiliation":[{"name":"Vaccine Process Research &amp; Development Merck &amp; Co., Inc.  West Point Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Winters","sequence":"additional","affiliation":[{"name":"Vaccine Process Research &amp; Development Merck &amp; Co., Inc.  West Point Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8841-8940","authenticated-orcid":false,"given":"Giorgio","family":"Carta","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,23]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Viruses: from Understanding to Investigation","author":"Payne S","year":"2017"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2006.06.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.01.002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2014.02.018"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2014.06.019"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.11.060"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.03.050"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2019.460578"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461079"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2021.462314"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/01496395.2022.2026388"},{"key":"e_1_2_9_13_1","unstructured":"Cytiva.Capto Core 400 and Capto Core 700 Multimodal Chromatography.2021."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2020.07.023"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2020.08.014"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.1960"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.03.008"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.06.072"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.04.077"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36826"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.12.035"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2018.05.007"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2016.02.011"},{"key":"e_1_2_9_24_1","first-page":"85","article-title":"A flow\u2010through chromatographic strategy for hepatitis C virus\u2010like particles purification","volume":"8","author":"Silva RJS","year":"2020","journal-title":"PRO"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2020.01.002"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1541396"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2020.122459"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.609880"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.12.052"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/biotech10040022"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15091"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2022.01.004"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22532"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-031413-085355"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1999.1155"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.19.1.95"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2016.118"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461444"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.12.007"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc049843w"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-4770(03)80026-X"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21080"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22145"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/aic.11191"},{"key":"e_1_2_9_45_1","volume-title":"Principles of Adsorption and Adsorption Processes","author":"Ruthven DM","year":"1984"},{"key":"e_1_2_9_46_1","unstructured":"Cytiva.Sepharose and Sepharose CL\u2010Size exclusion chromatography. 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