{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T19:53:37Z","timestamp":1778270017729,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T00:00:00Z","timestamp":1678147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["304678\/2021\u20100"],"award-info":[{"award-number":["304678\/2021\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["304680\/2021\u20104"],"award-info":[{"award-number":["304680\/2021\u20104"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This article developed a novel electrochemical immunosensor for the specific detection of aflatoxin B1 (AFB1). Amino\u2010functionalized iron oxide nanoparticles (Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    \u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    ) were synthesized. Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    \u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    were chemically bound on self\u2010assembly monolayers (SAMs) of mercaptobenzoic acid (MBA). Finally, polyclonal antibodies (pAb) were immobilized on Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    \u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    \u2010MBA. The sensor system was evaluated through atomic force microscopy (AFM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). A reduction in the anodic and cathodic peak currents was observed after the assembly of the sensor platform. The charge transfer resistance (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>ct<\/jats:sub>\n                    ) was increased due to the electrically insulating bioconjugates. Then, the specific interaction between the sensor platform and AFB1 blocks the electron transfer of the [Fe(CN)\n                    <jats:sub>6<\/jats:sub>\n                    ]\n                    <jats:sup>3\u2212\/4\u2212<\/jats:sup>\n                    redox pair. The nanoimmunosensor showed a linear response range estimated from 0.5 to 30\u2009\u03bcg\/mL with a limit of detection (LOD) of 9.47\u2009\u03bcg\/mL and a limit of quantification (LOQ) of 28.72\u2009\u03bcg\/mL for AFB1 identification in a purified sample. In addition, a LOD of 3.79\u2009\u03bcg\/mL, a LOQ of 11.48\u2009\u03bcg\/mL, and a regression coefficient of 0.9891 were estimated for biodetection tests on peanut samples. The proposed immunosensor represents a simple alternative, successfully applied in detecting AFB1 in peanuts, and therefore, represents a valuable tool for ensuring food safety.\n                  <\/jats:p>","DOI":"10.1002\/btpr.3334","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T09:49:37Z","timestamp":1676540977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4190-819X","authenticated-orcid":false,"given":"Gilcelia J. L. S.","family":"Barbieri","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biodispositivos Nanoestruturados, Departamento de Bioqu\u00edmica Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9020-4581","authenticated-orcid":false,"given":"Est\u00e9fani P.","family":"Sim\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biodispositivos Nanoestruturados, Departamento de Bioqu\u00edmica Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3002-0291","authenticated-orcid":false,"given":"Karen Y. P. S.","family":"Avelino","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biodispositivos Nanoestruturados, Departamento de Bioqu\u00edmica Universidade Federal de Pernambuco  Recife Pernambuco Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8572-4846","authenticated-orcid":false,"given":"Maria D. 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