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Especially, DNA in the form of chromatin is often neglected. Endonuclease treatment is an almost mandatory step in manufacturing of viral vaccines. In order to find the optimal performer, four different endonucleases, two of them salt tolerant, were evaluated in downstream processing of recombinant measles virus. Endonuclease treatment was performed under optimal temperature conditions after clarification and before the purification by flow\u2010through chromatography with a core shell chromatography medium: Capto\u2122 Core 700. Virus infectivity was measured by TCID50. DNA and histone presence in process and purified samples was determined using PicoGreen\u2122 assay and Western blot analysis using an anti\u2010histone antibody, respectively. All tested endonucleases allowed the reduction of DNA content improving product purity. The salt\u2010tolerant endonucleases SAN and M\u2010SAN were more efficient in the removal of chromatin compared with the non\u2010salt\u2010tolerant endonucleases Benzonase\u00ae and DENARASE\u00ae. Removal of chromatin using M\u2010SAN was also possible without the addition of extra salt to the cell culture supernatant. The combination of the endonuclease treatment, using salt\u2010tolerant endonucleases with flow\u2010through chromatography, using core\u2013shell particles, resulted in high purity and purification efficiency. This strategy has all features for a platform downstream process of recombinant measles virus and beyond.<\/jats:p>","DOI":"10.1002\/btpr.3342","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T01:42:25Z","timestamp":1679967745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Removal of chromatin by salt\u2010tolerant endonucleases for production of recombinant measles virus"],"prefix":"10.1002","volume":"39","author":[{"given":"Viktoria","family":"Mayer","sequence":"first","affiliation":[{"name":"acib \u2013 Austrian Centre of Industrial Biotechnology  Vienna Austria"},{"name":"Institute of Bioprocess Science and Engineering, Department of Biotechnology University of Natural Resources and Life Sciences  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna\u2010Carina","family":"Frank","sequence":"additional","affiliation":[{"name":"acib 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