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A more comprehensive and extensive metabolic pathway was obtained by optimization calculations. The primary extended branches include: the dihydroxyacetone node, which enters the pentose phosphate pathway; the \u03b1\u2010oxoglutarate node, which has synthetic metabolic pathways for glutamic acid and amino acids; and the serine and homocysteine nodes, which produce cystathionine before homocysteine enters the methionine cycle pathway. According to the expanded metabolic network, the flux distribution of key nodes in the metabolic pathway and the relationship between the flux distribution ratio of nodes and the yield of 1,3\u2010PDO were analyzed. At the dihydroxyacetone node, the flux of dihydroxyacetone converted to dihydroxyacetone phosphate was positively correlated with the yield of 1,3\u2010PDO. As an important intermediate product, the flux change in the metabolic pathway of \u03b1\u2010oxoglutarate reacting with amino acids to produce glutamic acid is positively correlated with the yield. When pyruvate was used as the central node to convert into lactic acid and \u03b1\u2010oxoglutarate, the proportion of branch flux was negatively correlated with the yield of 1,3\u2010PDO. These studies provide a theoretical basis for the optimization and further study of the metabolic pathway of\n                    <jats:italic>C. butyricum<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/btpr.3411","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T17:37:44Z","timestamp":1700588264000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Dynamic flux balance analysis of 1,3\u2010propanediol production by\n                    <i>clostridium butyricum<\/i>\n                    fermentation"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3648-1403","authenticated-orcid":false,"given":"Duo\u2010Tao","family":"Pan","sequence":"first","affiliation":[{"name":"Institute of Information and Engineering Shenyang University of Chemical and Technology  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Information and Engineering Shenyang University of Chemical and Technology  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Li","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science and Biotechnology Dalian University of Technology  Dalian PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Qin","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Life Science and Biotechnology Dalian University of Technology  Dalian PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1150-658X","authenticated-orcid":false,"given":"Zhi\u2010Long","family":"Xiu","sequence":"additional","affiliation":[{"name":"School of Life Science and Biotechnology Dalian University of Technology  Dalian PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1SE01333E"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-022-02143-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-63662-1.00031-2"},{"issue":"6","key":"e_1_2_9_5_1","first-page":"4","article-title":"The research Progress of 1,3\u2010Propanediol production methods","volume":"24","author":"Guishu Y","year":"2010","journal-title":"Chem Ind Times"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2012.05.012"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejlt.200800057"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13762-020-03036-w"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bbb.2254"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.9b04071"},{"issue":"29","key":"e_1_2_9_11_1","first-page":"7930","article-title":"Glycerol as a substrate and Inactivator of coenzyme B12\u2010dependent diol dehydratase. 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