{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T03:35:22Z","timestamp":1784691322937,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methylated tryptamines, such as the hallucinogenic natural products, psilocybin and\n                    <jats:italic>N,N<\/jats:italic>\n                    \u2010dimethyltryptamine (DMT), are gaining interest from the medical community due to their potential as next generation treatments for mental health disorders. The clinical relevance of these compounds has driven scientists to develop biosynthetic production routes to a number of tryptamine drug candidates, and efforts are ongoing to expand and further develop these biosynthetic capabilities. To that end, we have further characterized the substrate preferences of two enzymes involved in tryptamine biosynthesis: TrpM, a tryptophan\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyltransferase from\n                    <jats:italic>Psilocybe serbica<\/jats:italic>\n                    , and PsiD, the gateway decarboxylase of the psilocybin biosynthesis pathway. Here, we show that TrpM can\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methylate the non\u2010native amino acid substrate, 4\u2010hydroxytryptophan, a key intermediate in the\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    \u2010based recombinant psilocybin biosynthesis pathway. However, the ability to incorporate TrpM into a functional psilocybin biosynthesis pathway was thwarted by PsiD's inability to use\n                    <jats:italic>N,N<\/jats:italic>\n                    \u2010dimethyl\u20104\u2010hydroxytryptophan as substrate, under the culturing conditions tested, despite demonstrating activity on\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methylated and 4\u2010hydroxylated tryptophan derivatives individually. Taken together, this work expands upon the known substrates for TrpM and PsiD, further increasing the diversity of tryptamine biosynthetic products.\n                  <\/jats:p>","DOI":"10.1002\/btpr.3492","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T05:39:45Z","timestamp":1718689185000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of\n                    <scp>TrpM<\/scp>\n                    and\n                    <scp>PsiD<\/scp>\n                    substrate promiscuity reveals new biocatalytic capabilities"],"prefix":"10.1002","volume":"40","author":[{"given":"Fiona C.","family":"Kanis","sequence":"first","affiliation":[{"name":"Department of Chemical, Paper, and Biomedical Engineering Miami University  Oxford Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline N.","family":"Broude","sequence":"additional","affiliation":[{"name":"Department of Chemical, Paper, and Biomedical Engineering Miami University  Oxford Ohio USA"},{"name":"Department of Chemistry Williams College  Williamstown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elle B.","family":"Hellwarth","sequence":"additional","affiliation":[{"name":"Department of Chemical, Paper, and Biomedical Engineering Miami University  Oxford Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"William J.","family":"Gibbons","sequence":"additional","affiliation":[{"name":"Department of Chemical, Paper, and Biomedical Engineering Miami University  Oxford Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek K.","family":"Sen","sequence":"additional","affiliation":[{"name":"Department of Chemical, Paper, and Biomedical Engineering Miami University  Oxford Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra M.","family":"Adams","sequence":"additional","affiliation":[{"name":"Department of Chemical, Paper, and Biomedical Engineering Miami University  Oxford Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology Miami University  Oxford Ohio USA"},{"name":"Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9068-2126","authenticated-orcid":false,"given":"J. Andrew","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Chemical, Paper, and Biomedical Engineering Miami University  Oxford Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201705489"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2019.12.007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELREP.2021.109413"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.YMBEN.2019.09.009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.YMBEN.2018.05.014"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b01061"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mec.2022.e00196"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201900358"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28480"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/CBIC.201800336"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2023.107854"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202200551"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010016\u20100459\u2010x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.1987090"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/OL9910417"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/CBIC.201900069"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201308069"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2023.05.006"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.105231"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ANGE.201910175"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/CHEM.202000134"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2014.11.003"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.3492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T11:30:04Z","timestamp":1761564604000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.3492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,18]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/btpr.3492"],"URL":"https:\/\/doi.org\/10.1002\/btpr.3492","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,18]]},"assertion":[{"value":"2024-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e3492"}}