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Currently <jats:italic>t<\/jats:italic>CA is produced by industrial chemical synthesis from fossil fuels or cost\u2010prohibitive isolation from terrestrial plants. Cyanobacteria, a type of photosynthetic bacteria, can be readily engineered to convert sunlight and carbon dioxide into metabolites of interest at relatively high amounts compared to terrestrial plants. The purpose of this study is to advance the industrial and commercial value of cyanobacteria as a biological factory for renewable production of <jats:italic>t<\/jats:italic>CA. Production of <jats:italic>t<\/jats:italic>CA has previously been demonstrated in the model cyanobacterium <jats:italic>Synechocystis<\/jats:italic> sp. PCC 6803 (<jats:italic>S<\/jats:italic>. 6803) via expression of non\u2010native phenylalanine ammonia lyase (PAL) from various organisms. This project focuses on developing and characterizing a new high\u2010titer strain of <jats:italic>S.<\/jats:italic> 6803 expressing a plant PAL gene controlled by an inducible promoter. We assessed production in shake flasks under constant light, a 12\u2009h:12\u2009h\u2009light:dark cycle, and environmental photobioreactors (ePBRs) with a sinusoidal, rapidly fluctuating light environment. Our strain demonstrates a four\u2010fold increase in <jats:italic>t<\/jats:italic>CA production to ~500\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup> by 14\u2009days compared to previously reported titers in <jats:italic>S<\/jats:italic>. 6803 under shake flask cultivation and a 30\u201350% improved average <jats:italic>t<\/jats:italic>CA production per culture density (60\u2009mg\u00b7L<jats:sup>\u22121<\/jats:sup>\u00b7OD<jats:sub>730<\/jats:sub><jats:sup>\u22121<\/jats:sup>) in ePBRs over comparable previously reported culture methods. Our study progresses <jats:italic>S<\/jats:italic>. 6803 <jats:italic>t<\/jats:italic>CA bioproduction into higher culture volumes, up to 500\u2009mL, while further validating the strength of an inducible system for <jats:italic>t<\/jats:italic>CA production in <jats:italic>S.<\/jats:italic> 6803.<\/jats:p>","DOI":"10.1002\/btpr.3512","type":"journal-article","created":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T01:34:51Z","timestamp":1727832891000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Improving trans\u2010cinnamic acid production in a model cyanobacterium"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7477-7371","authenticated-orcid":false,"given":"Darcy","family":"Hunstiger","sequence":"first","affiliation":[{"name":"Cell and Molecular Biology Program Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayley","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Paton","sequence":"additional","affiliation":[{"name":"Department of Biology Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christie A. 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