{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:00:36Z","timestamp":1772982036964,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T00:00:00Z","timestamp":1268870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T00:00:00Z","timestamp":1268870400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In the production of ethanol from lignocellulosic material, pretreatment of the raw material before enzymatic hydrolysis and fermentation is essential to obtain high overall yields of sugar and ethanol. Two\u2010step steam pretreatment results in higher ethanol yields from softwood than the standard one\u2010step pretreatment process. However, the difficulty with separation and washing of the material at high pressure between the two pretreatment steps is a major drawback. In this study, a new one\u2010step pretreatment procedure was investigated, in which the time\u2010temperature profile was varied during pretreatment. The efficiency of pretreatment was assessed by performing simultaneous saccharification and fermentation on the pretreated slurries. Pretreatment of SO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010impregnated softwood performed by varying the temperature (190\u2013226\u00b0C), the residence time (5\u201310 min), and the mode of temperature increase (linear or stepwise), resulted in recovery of about 90% of the mannose and glucose present in the raw material. The highest ethanol yield, 75% of theoretical based on the glucan and mannan content of the raw material, was obtained at pretreatment conditions of 190\u00b0C for 12 min. Similar ethanol yields were achieved when running the pretreatment as one\u2010step (190\u2013200\u00b0C), two levels of temperature, at shorter residence time (7 min), which results in lower capital costs for the process. \u00a9 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010\n                  <\/jats:p>","DOI":"10.1002\/btpr.421","type":"journal-article","created":{"date-parts":[[2010,3,20]],"date-time":"2010-03-20T18:46:11Z","timestamp":1269110771000},"page":"1054-1060","source":"Crossref","is-referenced-by-count":23,"title":["Improved one\u2010step steam pretreatment of SO\n                    <sub>2<\/sub>\n                    \u2010Impregnated softwood with time\u2010dependent temperature profile for ethanol 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