{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:14:48Z","timestamp":1789989288408,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,4,9]],"date-time":"2010-04-09T00:00:00Z","timestamp":1270771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To prevent protein aggregation, some proteins are usually expressed as fusion proteins from which target proteins can be released by proteolytic or chemical reagents. In this report, small ubiquitin\u2010related modifier (SUMO) linked with a hexa\u2010histidine tag was used as a fusion partner for the antitumor\u2010analgesic peptide from the venom of <jats:italic>Buthus martensii<\/jats:italic> (Karsch) scorpion (AGAP). The optimal expression level of the soluble fusion protein, SUMO\u2010AGAP, was up to 40% of the total cellular protein. The fusion protein was purified by Ni\u2010NTA affinity chromatography and cleaved by a SUMO\u2010specific protease (Ulp1) to obtain the recombinant AGAP (rAGAP), which was further purified by Ni\u2010NTA affinity chromatography. The purified final product was &gt;95% pure by SDS\u2010PAGE stained with Coomassie brilliant blue R\u2010250. Mass spectroscopic analysis indicated the protein to be 7142.63 Dalton, which equaled the theoretically expected mass. N\u2010terminal sequencing of rAGAP showed the sequence corresponded to the native protein. MTT assay indicated the rAGAP could significantly inhibit the proliferation of Jurkat and Hut 78 T lymphoma cell lines. The further writhing experiment showed that the rAGAP had an intensive analgesic effect. The expression strategy presented in this study allows convenient high yield and easy purification of the rAGAP with native sequences. \u00a9 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010<\/jats:p>","DOI":"10.1002\/btpr.433","type":"journal-article","created":{"date-parts":[[2010,4,12]],"date-time":"2010-04-12T14:05:35Z","timestamp":1271081135000},"page":"1240-1244","source":"Crossref","is-referenced-by-count":22,"title":["Expression and purification of an antitumor\u2010analgesic peptide from the venom of <i>Mesobuthus martensii<\/i> Karsch by small ubiquitin\u2013related modifier fusion in <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"26","author":[{"given":"Peng","family":"Cao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiemiao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuguang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueting","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingmei","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,4,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.5.981"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.23.10391-10406.2005"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307533200"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0637114100"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.1839"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.04.076"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.03.016"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(64)90030-3"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.20.040180.000311"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/296090a0"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/313316a0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.2.C361"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-5928(02)00609-5"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.11.026"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-008-9277-5"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(97)01155-4"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-006-0673-2"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.23.120189.001115"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0958-1669(95)80083-2"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1997.0767"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00139-6"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.02.004"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbtpr.433","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.433","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:53:46Z","timestamp":1760046826000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,9]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/btpr.433"],"URL":"https:\/\/doi.org\/10.1002\/btpr.433","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,9]]}}}