{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:38:25Z","timestamp":1790289505826,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2021QB176"],"award-info":[{"award-number":["ZR2021QB176"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Redesigning metabolic pathways to enhance the efficiency of carbon fixation during chemical biosynthesis is a promising approach for achieving cleaner and greener production of multi\u2010carbon compounds. In this study, we established a model of cell growth in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    that is dependent on the RuBisCO\u2010Prk pathway by regulating its central metabolism. This rewiring ensures that growth depends on RuBisCO's carboxylation, allowing heterotrophic growth to rely on carbon fixation. This model was verified by detecting the growth curve, and it was used to screen four RuBisCO genes, of which the gene from\n                    <jats:italic>Rhodospirillum rubrum<\/jats:italic>\n                    ATCC 11170 serves as a growth advantage for\n                    <jats:italic>E.coli<\/jats:italic>\n                    . In addition, this model was applied to construct an efficient succinate biosynthetic pathway that can produce two moles of succinate from one mole of xylose and three moles of CO\n                    <jats:sub>2<\/jats:sub>\n                    . Compared to conventional succinate biosynthesis, this strategy has a CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation capacity that is 1.5 times greater. Furthermore, to optimize succinate production, various approaches were employed, including the optimization of key enzymes, substrate transport, and the supply of inorganic carbon. The resulting strain was capable of producing succinate at a level of 2.09\u2009\u00b1\u20090.14\u2009g\/L, which is nearly 22.4 times that of the original strain. In conclusion, this study was developed for the production of two moles of succinate by implementing three moles of carbon fixation reactions and demonstrated the feasibility of various optimization strategies in biological carbon fixation.\n                  <\/jats:p>","DOI":"10.1002\/btpr.70015","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T05:10:28Z","timestamp":1739941828000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The production of succinate with more\n                    <scp>\n                      CO\n                      <sub>2<\/sub>\n                    <\/scp>\n                    fixation reactions facilitated by\n                    <scp>RuBisCO<\/scp>\n                    \u2010based engineered\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"41","author":[{"given":"Xiuyuan","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Life Sciences and Medicine Shandong University of Technology  Zibo China"},{"name":"International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis Shandong University of Technology  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linqing","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine Shandong University of Technology  Zibo China"},{"name":"International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis Shandong University of Technology  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengjie","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine Shandong University of Technology  Zibo China"},{"name":"International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis Shandong University of Technology  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine Shandong University of Technology  Zibo China"},{"name":"International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis Shandong University of Technology  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5770-4461","authenticated-orcid":false,"given":"Xiutao","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine Shandong University of Technology  Zibo China"},{"name":"International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis Shandong University of Technology  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal1559"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-019-0421-5"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.synbio.2023.12.006"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010016\u20100530\u20107"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068\u2010015\u20100268\u20101"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2018.05.007"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0908356106"},{"issue":"1","key":"e_1_2_9_9_1","first-page":"44","article-title":"Establishing carbon dioxide\u2010based third\u2010generation biorefinery for a sustainable low\u2010carbon economy","volume":"1","author":"Shi 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