{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:59:33Z","timestamp":1772992773848,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL138116"],"award-info":[{"award-number":["R01HL138116"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL156526"],"award-info":[{"award-number":["R01HL156526"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL158076"],"award-info":[{"award-number":["R01HL158076"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL159862"],"award-info":[{"award-number":["R01HL159862"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL162120"],"award-info":[{"award-number":["R01HL162120"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Plasma expanders (PEs) are commonly used to replace lost blood volume for septic shock patients with increased vascular permeability. Human serum albumin (HSA) is the preferred PE, due to its innate ability to restore blood colloid osmotic pressure (COP). However, HSA is susceptible to protein extravasation under endothelial dysfunction leading to edema and exposing tissue to toxic HSA\u2010bound metabolites. To prevent extravasation, the molecular diameter of HSA has been previously increased through chemical polymerization to yield polymerized HSA\u00a0(PHSA). In this study, we further optimize PHSA size and COP via polyethylene glycol (PEG) surface conjugation. Previously synthesized PHSA that was size fractionated via tangential flow filtration (TFF) into two brackets (bracket A [500\u2009kDa\u20130.2\u2009\u03bcm] and bracket B [50\u2013500\u2009kDa]) served as precursors for subsequent PEGylation. Each PHSA bracket was thiolated with 2\u2010iminothiolane hydrochloride (IT) and PEGylated with monofunctional 5\u2009kDa maleimide PEG to yield PEGylated PHSA (PPHSA). All PPHSA solutions exhibited increased molecular size, zeta potential, and osmolality compared to their non\u2010PEGylated precursor PHSA. At the same total protein concentration, PPHSA viscosity decreased compared to the precursor PHSA, while the COP remained consistent with HSA, indicating their potential to serve as PEs.<\/jats:p>","DOI":"10.1002\/btpr.70054","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T05:39:33Z","timestamp":1752557973000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>PEGylation<\/scp>\n                    of polymerized albumin retains colloid osmotic pressure: Towards an enhanced potential plasma substitute"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2701-4547","authenticated-orcid":false,"given":"Amna","family":"Abdalbaqi","sequence":"first","affiliation":[{"name":"William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Yahya","sequence":"additional","affiliation":[{"name":"William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krianthan","family":"Govender","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gala Sanchez","family":"Van Moer","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Lucas","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Cabrales","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre F.","family":"Palmer","sequence":"additional","affiliation":[{"name":"William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/2110\u20105820\u20103\u20104"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23006"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc1332"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/536852"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/shk.0000000000000473"},{"issue":"2","key":"e_1_2_10_7_1","first-page":"765","article-title":"Cellular and molecular mechanisms of endothelial ischemia\/reperfusion injury: perspectives and implications for postischemic myocardial protection","volume":"8","author":"Yang Q","year":"2016","journal-title":"Am J Transl Res"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.16462"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000167111.80753.ef"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/nan.0000000000000103"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/aas.13603"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00733"},{"issue":"6","key":"e_1_2_10_13_1","first-page":"565","article-title":"EVIDENCE OF TRANSCELLULAR ALBUMIN TRANSPORT after HEMORRHAGIC SHOCK","volume":"23","author":"Childs EW","year":"2005","journal-title":"Shock"},{"key":"e_1_2_10_14_1","volume-title":"Capillary Fluid Exchange: Regulation, Functions, and Pathology","author":"Scallan J","year":"2009"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4377-0167-8.00022-4"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.531"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3233\/bir\u20102012\u20100598"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00117.2021"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201090431\u2010z"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep04871"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.3500"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajem.2012.05.031"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.15171\/apb.2016.043"},{"key":"e_1_2_10_24_1","unstructured":"ThermoS.TNBSA (2 4 6\u2010trinitrobenzene sulfonic acid).2008https:\/\/www.thermofisher.com\/document\u2010connect\/document\u2010connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS\u2010Assets%2FLSG%2Fmanuals%2FMAN0011392_TNBSA_UG.pdf&title=VXNlciBHdWlkZTogIFROQlNB(accessed 2024 07\/31)"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/358209a0"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.5b00143"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm500246w"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2161"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b01451"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201500294"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201200325"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ces.2015.07.031"},{"issue":"4","key":"e_1_2_10_33_1","first-page":"735","article-title":"PEGylation of nanoparticles improves their cytoplasmic transport","volume":"2","author":"Suh J","year":"2007","journal-title":"Int J Nanomedicine"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930\u2010006\u20109010\u2010y"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20050663"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3109\/10731199.2011.579568"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.70054","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/btpr.70054","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.70054","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:14:44Z","timestamp":1772979284000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.70054"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,14]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/btpr.70054"],"URL":"https:\/\/doi.org\/10.1002\/btpr.70054","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,14]]},"assertion":[{"value":"2025-03-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70054"}}