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Culture pH has been shown to be a critical parameter that influences growth, productivity, and critical quality attributes. Typically, pH is either controlled to a set\u2010point throughout the culture or uses a single pH shift to achieve higher productivity and more desirable charge variant profiles. The pH is usually maintained by CO\n                    <jats:sub>2<\/jats:sub>\n                    and base additions. For CO\n                    <jats:sub>2<\/jats:sub>\n                    controlled cultures, using a set\u2010point can result in an accumulation of CO\n                    <jats:sub>2<\/jats:sub>\n                    , which has detrimental effects on mammalian cell growth and protein production. In this study, a dynamic pH profile was implemented that allowed the pH control in the bioreactor to mimic the natural uncontrolled pH profile observed in shake flask cultures. This dynamic pH profile employs multiple pH shifts during the exponential phase of a single IgG\n                    <jats:sub>1<\/jats:sub>\n                    producing CHO\u2010K1 cell line. The results show that a dynamic pH profile was able to successfully alleviate CO\n                    <jats:sub>2<\/jats:sub>\n                    accumulation and increase the cell\u2010specific, as well as overall culture productivity. Impacts of the dynamic pH profile on product quality attributes, including glycosylation and charge variants, were also evaluated, showing mixed impacts on the glycosylation pattern and a positive impact on charge variants. Since the ideal glycosylation pattern is highly dependent on the intended function of the recombinant antibody, impacts on product quality should be evaluated on a \u201cper process\u201d basis.\n                  <\/jats:p>","DOI":"10.1002\/btpr.70080","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T02:10:01Z","timestamp":1761012601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dynamic\n                    <scp>pH<\/scp>\n                    profiles drive higher cell\u2010specific and volumetric productivity"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7794-5680","authenticated-orcid":false,"given":"Stephanie R.","family":"Klaubert","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Clemson University  Clemson South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan G.","family":"Chitwood","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Clemson University  Clemson South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danqia","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Tulane University  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Redman","sequence":"additional","affiliation":[{"name":"Department of Research and Development 908 Devices, Inc.  Morrisville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Young L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Research and Development 908 Devices, Inc.  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas R.","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Tulane University  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9417-0489","authenticated-orcid":false,"given":"Sarah W.","family":"Harcum","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Clemson University  Clemson South Carolina 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