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This study identified limitations in minimum residence time (mRT) prediction for packed bed reactors (PBR) utilized for CVI. This work focused on characterizing the residence time distribution (RTD) behavior of tracers with varying molecular properties in four PBR configurations. The results demonstrated that tracer molecular size impacted mRT prediction, with larger molecules showing shorter residence times than smaller molecule tracers under identical conditions. During scale\u2010up from 16 to 26\u2009mm diameter columns, mRT was not maintained, suggesting that traditional chromatography scale\u2010up principles may not be directly applicable to CVI using PBRs. 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