{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:59:19Z","timestamp":1791197959833,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bi\u2010 and multispecific antibodies can deliver effective protein\u2010based therapeutics by enabling novel biology. Transient expression of these complex asymmetric antibody formats may become a bottleneck in providing sufficient amounts of high\u2010quality material for preclinical discovery and developability assessment to support candidate selection activities. In contrast, transposase\u2010based stable cell line generation is being recognized as a robust and rapid path to enable the evaluation of product quality from stable pool derived material. Transposase\u2010based systems feature a semi\u2010targeted integration of multiple copies of the gene(s) of interest in the host cell's genome resulting in the rapid generation of homogeneous high\u2010expressing (up to g\/L range) stable pools after 1\u20133\u2009weeks of selection phase. Here, we evaluated the Leap\u2010In\u00ae transposase technology for the expression of IGI's 1\u2009+\u20091 and 2\u2009+\u20091 bispecific, common light chain antibodies, based on IGI's proprietary BEAT\u00ae (Bispecific Engagement of Antibodies based on T cell Receptor) platform. To generate stable CHO pools expressing IGI's bispecific molecules, transposon\u2010based constructs expressing the common light chain and the two heavy chain genes were co\u2010transfected with the Leap\u2010In\u00ae transposase mRNA. Based on the proportion of the different chain combinations detected in the harvests, their expression levels were further optimized through vector engineering to facilitate heterodimer assembly and maximize productivity. The best pool displayed a titer of 2.33\u2009g\/L in 14\u2010day fed\u2010batch cultures, and heterodimer represented 93.8% of the expressed species. The purified antibody displayed 97.4% purity, as determined by SE\u2010HPLC and binding to targets within range, as determined by SPR and cell\u2010based affinity.<\/jats:p>","DOI":"10.1002\/btpr.88517","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:24:52Z","timestamp":1780453492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation of high\u2010expressing transposon\u2010based stable pools to produce (1\u2009+\u20091) and (2\u2009+\u20091) bispecific common light chain antibodies"],"prefix":"10.1002","volume":"42","author":[{"given":"Sara","family":"Ramos","sequence":"first","affiliation":[{"name":"Ichnos Glenmark Innovation (IGI) Therapeutics Antibody Discovery and Engineering Department  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surya","family":"Karunakaran","sequence":"additional","affiliation":[{"name":"ATUM Product Management Department  Newark California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Monney","sequence":"additional","affiliation":[{"name":"Ichnos Glenmark Innovation (IGI) Therapeutics Antibody Discovery and Engineering Department  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Bornert","sequence":"additional","affiliation":[{"name":"Ichnos Glenmark Innovation (IGI) Therapeutics Antibody Discovery and Engineering Department  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Reimann","sequence":"additional","affiliation":[{"name":"Ichnos Glenmark Innovation (IGI) Therapeutics Antibody Discovery and Engineering Department  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferenc","family":"Boldog","sequence":"additional","affiliation":[{"name":"ATUM Cell Line Development Department  Newark California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Dyson","sequence":"additional","affiliation":[{"name":"Ichnos Glenmark Innovation (IGI) Therapeutics Antibody Discovery and Engineering Department  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Ichnos Glenmark Innovation (IGI) Therapeutics Antibody Discovery and Engineering Department  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Minshull","sequence":"additional","affiliation":[{"name":"ATUM  Newark California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6302-3453","authenticated-orcid":false,"given":"Lydia N.","family":"Caro","sequence":"additional","affiliation":[{"name":"Ichnos Glenmark Innovation (IGI) Therapeutics Antibody Discovery and Engineering Department  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202114291"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020\u2010018\u20100051\u20104"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2022.2153410"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2018.02.004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2018.11.008"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/9.7.617"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1220145110"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2018.07.014"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m117.782433"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2019.1660564"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.27742"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2022.108683"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/gen.39.09.18"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201700748"},{"key":"e_1_2_10_16_1","doi-asserted-by":"crossref","unstructured":"RajendranS KottayilI WebsterL et al.Cell line development for bispecific antibodies: better predictability through transposases.bioRxiv. 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