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To improve harvest and purification processes, the development of continuous membrane\u2010based operations, such as normal flow filtration (NFF) and single pass tangential flow filtration (SPTFF) for concentration were explored. This work was conducted using two model viruses, non\u2010enveloped porcine parvovirus (PPV) and enveloped Suid herpesvirus (SuHV). The viruses are in the same family as the gene therapy vectors adeno associated virus and herpes simplex virus, respectively. SPTFF design started with batch TFF for membrane selection. Hollow fiber membranes with a 100 and 300\u2009kDa molecular weight cut off were defined for PPV and SuHV SPTFF operations, respectively. The SPTFF runs for PPV did not provide any concentration of the virus and low protein and DNA removal, unlike batch TFF. Two hollow fiber membranes run at 10\u2009mL\/min and 2\u2009psi were the best condition for SuHV concentration, with approximately 100\u2010fold titer concentration and protein and DNA removal of 37%\u2009\u00b1\u20095% and 32%\u2009\u00b1\u20098%, respectively. This concentration was superior to the batch TFF and indicated a strong dependence on flow rate and transmembrane pressure. For NFF, filters selection and performance tests were carried out for NFF of PPV and SuHV, as well as cleaning protocols for hollow fiber membranes. The ultimate goal is to integrate this work into the continuous purification of viral vectors produced in mammalian cell cultures to reduce costs and increase throughput.<\/jats:p>","DOI":"10.1002\/btpr.88521","type":"journal-article","created":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T07:57:18Z","timestamp":1783151838000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Purification and concentration of model viruses using single\u2010pass tangential flow filtration"],"prefix":"10.1002","volume":"42","author":[{"given":"Luis Alberto","family":"Mej\u00eda\u2010Manzano","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Michigan Technological University  Houghton Michigan USA"},{"name":"School of Engineering and Sciences Tecnologico de Monterrey  Monterrey 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