{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T07:23:19Z","timestamp":1787383399507,"version":"build-2736575974"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    N\u2010glycosylation is a key post\u2010translational modification of proteins influencing their physicochemical properties as well as biological activity. This study investigates the\n                    <jats:italic>in vivo<\/jats:italic>\n                    activity of four \u03b2\u20101,4\u2010galactosyltransferase 1 inhibitors in cell cultures producing recombinant IgG antibodies. Inhibitors, previously identified as potent inhibitors in on\u2010target assay, were evaluated in fed\u2010batch bioprocesses. Dose\u2010dependent effects on cell culture performance were assessed following compound addition. N\u2010glycan profiles were analyzed using InstantPC labeling and UHPLC\u2010FLD, while intracellular compound concentrations were quantified via LC\u2013MS\/MS following time\u2010resolved sampling of treated cell lysates. Bioprocess parameters, including cell growth, viability, and productivity, were monitored throughout cultivation. The study revealed divergent behaviors between on\u2010target potency and\n                    <jats:italic>in vivo<\/jats:italic>\n                    activity, underscoring the importance of cellular permeability, metabolic stability, and off\u2010target effects of potential inhibitors used in cell culture. These findings provide new insights into the challenges of glycosylation pathway modulation and inform strategies for future glycoengineering tool development.\n                  <\/jats:p>","DOI":"10.1002\/btpr.88522","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T09:16:41Z","timestamp":1779355001000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\u03b2\u20101,4\u2010galactosyltransferase 1 inhibitors modulate cellular N\u2010glycan profiles"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8218-9881","authenticated-orcid":false,"given":"Jaka","family":"Kranjc","sequence":"first","affiliation":[{"name":"Institute for Pharmacy, Faculty of Pharmacy University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stane","family":"Pajk","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy, University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1768-8246","authenticated-orcid":false,"given":"Marko","family":"Anderluh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy, University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1159-1024","authenticated-orcid":false,"given":"Anja","family":"Pi\u0161lar","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology Faculty of Pharmacy, University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matja\u017e","family":"Brinc","sequence":"additional","affiliation":[{"name":"Bioprocess Development, Technical Development Biologics Novartis Technical Research &amp; Development, Novartis LLC  Menge\u0161 Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Essentials of Glycobiology","author":"Stanley P","year":"2022"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwv065"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2018.11.017"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2014.11.001"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/11.10.813"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-020-09941-z"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2020.04.017"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.19868"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00397910902730887"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(98)00618-0"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/9781849737173-00078"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2024.1448925"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.202400896"},{"key":"e_1_2_10_15_1","unstructured":"Agilent Technologies.Agilent AdvanceBio Gly\u2010X N\u2010Glycan Prep with InstantPC Kit 96\u2010ct (formerly Prozyme).2023."}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.88522","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/btpr.88522","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.88522","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T06:33:22Z","timestamp":1787380402000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.88522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,21]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/btpr.88522"],"URL":"https:\/\/doi.org\/10.1002\/btpr.88522","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,21]]},"assertion":[{"value":"2026-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e88522"}}