{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:59:48Z","timestamp":1791197988637,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1841474"],"award-info":[{"award-number":["1841474"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2310832"],"award-info":[{"award-number":["2310832"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>High performance countercurrent membrane purification (HPCMP) has emerged as a promising diffusive separation strategy for the purification of recombinant proteins and monoclonal antibodies (mAbs). However, prior studies employed dialysis membranes, which are designed for diffusive separations but have not been validated for biopharmaceutical manufacturing. This study evaluates the ability of commercial ultrafiltration (UF) membranes, engineered for pressure\u2010driven filtration, to support highly selective diffusive separations. Scanning electron microscopy (SEM) analysis of the membrane cross\u2010sections revealed marked morphological differences between UF and dialysis membranes. Yet, transport studies using polydisperse dextrans and model proteins demonstrated that UF membranes can deliver exceptional diffusive separation selectivity. In particular, a 100\u2009kDa polysulfone membrane (Cytiva) provided &gt;150\u2010fold diffusive selectivity between bovine serum albumin (BSA) and immunoglobulin G (IgG). When utilized for mAb separation from a clarified CHO cell culture fluid, this membrane achieved &gt;98% product yield, near\u2010complete removal of low molecular weight species, and significant reduction in both DNA and host cell protein (HCP) levels. These results demonstrate that commercial UF membranes that are validated for bioprocessing applications can be repurposed for HPCMP, enabling scalable, continuous downstream processing of biotherapeutics.<\/jats:p>","DOI":"10.1002\/btpr.88537","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T03:37:02Z","timestamp":1783481822000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (\n                    <scp>HPCMP<\/scp>\n                    )"],"prefix":"10.1002","volume":"42","author":[{"given":"Aylin","family":"Mohammadzadehmarandi","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nate","family":"Heller","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadreza","family":"Ahmadimashhadi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon","family":"Petrone","sequence":"additional","affiliation":[{"name":"Cytiva  Marlborough Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Marchand","sequence":"additional","affiliation":[{"name":"Cytiva  Marlborough Massachusetts 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/BTT.S515078"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2024.10.007"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(25)00481\u20105"},{"key":"e_1_2_10_5_1","unstructured":"WHO.World malaria report 2024.2025https:\/\/www.who.int\/teams\/global-malaria-programme\/reports\/world-malaria-report-2024"},{"key":"e_1_2_10_6_1","unstructured":"Gates Foundation https:\/\/gcgh.grandchallenges.org\/article\/grand\u2010challenges\u2010announces\u2010awards\u2010innovations\u2010exceptionally\u2010low\u2010cost\u2010monoclonal\u2010antibody 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