{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T07:14:04Z","timestamp":1788246844969,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Recombinant adeno\u2010associated virus production for gene therapy applications commonly relies on the\n                    <jats:italic>Spodoptera frugiperda<\/jats:italic>\n                    9 (Sf9) baculovirus infection platform. The process encompasses cell expansion from flasks through intermediate\u2010scale bioreactors to large\u2010scale production. A critical challenge in this process is suboptimal cell proliferation during the expansion phase, which significantly impacts subsequent virus production. This study evaluates the effects of different single\u2010use bioreactor bags on Sf9 cell growth, focusing on two potential issues: incompatibility between Sf9 cells and bag materials, and the presence of leachables and extractables from the bag. Single\u2010use rocking motion bioreactor bag configuration, including material\u2010 and surface\u2010related attributes, was associated with substantial differences in Sf9 cell expansion performance. While growth kinetics in shake flasks and the Pall Allegro XRS system were comparable, reduced growth rates and delayed proliferation were observed in Cytiva Cellbags. The comparable growth observed in shake flasks and the Pall XRS system, together with the lack of recovery after rocking\u2010parameter adjustments in Cellbags, suggests that bag\u2010associated material and surface\u2010related factors, rather than rocking motion alone, contributed substantially to the observed growth differences. Lower bag occupancy further exacerbated growth inhibition. Washing studies showed no improvement in new bags, whereas partial recovery after initial use suggested transient bag conditioning or surface\u2010related effects. Analytical extractables and leachables profiling was not performed; therefore, leachable\u2010mediated contributions cannot be fully excluded. These findings highlight the importance of single\u2010use bag selection and occupancy optimization for robust and scalable Sf9 cell expansion processes.\n                  <\/jats:p>","DOI":"10.1002\/btpr.88554","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:30:01Z","timestamp":1788244201000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of single\u2010use bioreactor bag materials on\n                    <i>Spodoptera frugiperda<\/i>\n                    cell expansion for recombinant adeno\u2010associated virus manufacturing"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4644-362X","authenticated-orcid":false,"given":"Min Tae","family":"Park","sequence":"first","affiliation":[{"name":"Viral Vector Services Thermo Fisher Scientific  Plainville Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3768-789X","authenticated-orcid":false,"given":"Seyed Pouria","family":"Motevalian","sequence":"additional","affiliation":[{"name":"Viral Vector Services Thermo Fisher Scientific  Plainville Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2025.02.016"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2012.10.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010009\u20102422\u20109"},{"key":"e_1_2_9_5_1","first-page":"3","volume-title":"Biopharmaceutical Manufacturing: Progress, Trends and Challenges","author":"Jossen V","year":"2024"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010chembioeng\u2010092220\u2010030223"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/9781119477891.ch4","volume-title":"Single\u2010Use Technology in Biopharmaceutical Manufacture","author":"Jossen V","year":"2019"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201074954\u20109"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2024.109428"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b01208"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.2322"},{"issue":"3","key":"e_1_2_9_12_1","first-page":"26","article-title":"Evaluating new film for single\u2010use bags: growth performance studies with animal and human cells","volume":"14","author":"Blaschczock K","year":"2016","journal-title":"Bioprocess Int"},{"key":"e_1_2_9_13_1","unstructured":"MannK MaherM PerezD KumpeyP SilvaD DupontA.Single\u2010use upstream processing: Ultimus\u00ae film delivers comparable cell growth performance to glass. [Millipore White Paper 10 January 2026]. [Online]. Available:https:\/\/www.sigmaaldrich.com\/deepweb\/assets\/sigmaaldrich\/marketing\/global\/documents\/134\/272\/ultimus\u2010film\u2010cell\u2010growth\u2010wp\u2010an9607en\u2010ms.pdf?srsltid=AfmBOoro\u2010ZEhwWumYRyHlrhVnlFk4_dp_WeUNbQJ5BXUNoKkFE7eYnxa"},{"key":"e_1_2_9_14_1","unstructured":"FaganS.Xcellerex\u2122 XDR bioreactor single\u2010use bag film comparison study. [Cytiva Technical Report 13 January 2026]. [Online]. Available:https:\/\/www.cytivalifesciences.com\/en\/us\/knowledge\u2010center\/xcellerex\u2010xdr\u2010bioreactor\u2010single\u2010use\u2010bag\u2010film\u2010comparison\u2010study"},{"issue":"8","key":"e_1_2_9_15_1","first-page":"S34","article-title":"Consistently superior cell growth: achieved with new polyethylene film formulation","volume":"12","author":"Fenge C","year":"2014","journal-title":"Bioprocess Int"},{"key":"e_1_2_9_16_1","unstructured":"LugariA SchenkT.Cell growth performance in single\u2010use bags. [Sartorius White Paper 10 January 2026]. [Online]. Available:https:\/\/www.sartorius.com\/download\/13284\/white\u2010paper\u2010cellgrowth\u2010flexsafe\u2010flexboy\u2010spt1103\u2010e\u2010data.pdf"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/10_2008_15","volume-title":"Disposable Bioreactors","author":"Eibl R","year":"2009"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529\u2010012\u20100915\u20106"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1263\/jbb.103.50"},{"issue":"1","key":"e_1_2_9_20_1","first-page":"22","article-title":"Analyzing single\u2010use polymers for cell culture processes: comparison of cell growth and viability test procedures","volume":"20","author":"Kneitz J","year":"2022","journal-title":"BioProcess Int"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2020.09.018"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2025.1679358"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021102015070"},{"key":"e_1_2_9_24_1","unstructured":"KaiserSC PerepelitsaN.Cultivation of Sf9 insect cells and rSEAP expression in the HyPerforma Rocker Bioreactor. [Thermo Scientific Application Note 10 January 2026]. [Online]. Available:https:\/\/assets.thermofisher.com\/TFS\u2010Assets\/BPD\/Application\u2010Notes\/cultivation\u2010Sf9\u2010insect\u2010cells\u2010seap\u2010expression\u2010hyp\u2010rocker\u2010bioreactor\u2010app\u2010note.pdf"},{"key":"e_1_2_9_25_1","unstructured":"RathodAK DiwakarS TappeA HerrmannJ AdamsT MundrigiA.Cultivation of Sf9 cells in the single use bioreactor BIOSTAT\u00ae RM 20|50 optical system. [Sartorius Application Note 10 January 2026]. [Online]. Available:https:\/\/sartorius.ba\/media\/vx4m1wlb\/appl-cultivation-of-sf9-cells-with-biostat-rm-sbt1014-e-data.pdf"},{"issue":"5","key":"e_1_2_9_26_1","first-page":"40","article-title":"Large\u2010scale, insect\u2010cell\u2010based vaccine development: cell banking, one\u2010step inoculation, and repeated fed\u2010batch expansion in a single\u2010use bioreactor","volume":"10","author":"B\u00f6gli NC","year":"2012","journal-title":"BioProcess Int"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.201300131"},{"issue":"8","key":"e_1_2_9_28_1","first-page":"S38","article-title":"Robust and convenient single\u2010use processing: the superior strength and flexibility of Flexsafe bags.","volume":"12","author":"Vachette E","year":"2014","journal-title":"BioProcess International"},{"key":"e_1_2_9_29_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1201\/b11472-5","volume-title":"Disposable Bioprocessing Systems","author":"Niazi SK","year":"2016"},{"key":"e_1_2_9_30_1","unstructured":"Cytiva.Bioclear\u2122 10 and 11 films are comparable. [Cytiva Technical Report 4 January 2026]. [Online]. Available:https:\/\/www.cytivalifesciences.com\/en\/us\/solutions\/bioprocessing\/knowledge\u2010center\/bioclear\u201010\u2010and\u201011\u2010films\u2010are\u2010comparable"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.88554","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/btpr.88554","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.88554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:30:06Z","timestamp":1788244206000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.88554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":29,"alternative-id":["10.1002\/btpr.88554"],"URL":"https:\/\/doi.org\/10.1002\/btpr.88554","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]},"assertion":[{"value":"2026-06-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e88554"}}