{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T06:08:02Z","timestamp":1788761282080,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL158076"],"award-info":[{"award-number":["R01HL158076"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL159862"],"award-info":[{"award-number":["R01HL159862"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL162120"],"award-info":[{"award-number":["R01HL162120"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014055","name":"U.S. Army Medical Research Acquisition Activity","doi-asserted-by":"publisher","award":["HT94252410238"],"award-info":[{"award-number":["HT94252410238"]}],"id":[{"id":"10.13039\/100014055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Haptoglobin (Hp) is a polymorphic acute phase\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u20102 glycoprotein found in plasma that plays a critical role in binding, neutralizing, and removing cell\u2010free hemoglobin (Hb) from the circulation. Under clinical conditions characterized by high levels of hemolysis, such as in patients with sickle cell disease (SCD), large quantities of cell\u2010free Hb are released from lysed red blood cells (RBCs) into the circulation and bind to Hp. This interaction reduces the plasma Hp concentration below basal levels and diminishes its Hb\u2010binding capacity. Therefore, plasma\u2010derived Hp has the potential to be used therapeutically to scavenge, neutralize, and remove excess cell\u2010free Hb from the blood, thus preventing Hb\u2010mediated toxicity. This provides strong motivation to purify Hp at high purity using sustainable sources, such as waste plasma fractions from the Cohn plasma fractionation process used to produce human serum albumin from pooled plasma. Starting from human Cohn fraction IV, we first enriched an Hp\u2010rich fraction using tangential flow filtration (TFF), which was then used as the starting material for purification. Hp was subsequently purified from this fraction using hydrophobic interaction chromatography (HIC) to homogeneity. The final Hp purity reached 98% by SDS\u2010PAGE densitometry and 87% by trypsin digest LC\u2013MS\/MS analysis. The purified Hp was further characterized to determine its molecular weight, secondary structure, Hb\u2010binding capacity, and binding kinetics using biophysical techniques including MALDI\u2010TOF, circular dichroism, size exclusion HPLC, and UV\u2013visible stopped\u2010flow spectroscopy. More importantly, the simplicity and efficiency of the TFF\u2013HIC workflow demonstrated strong potential for scalable Hp production.\n                  <\/jats:p>","DOI":"10.1002\/btpr.88555","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T05:26:40Z","timestamp":1788758800000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Small scale purification and biophysical characterization of haptoglobin derived from human Cohn fraction\n                    <scp>IV<\/scp>"],"prefix":"10.1002","author":[{"given":"Shuwei","family":"Lu","sequence":"first","affiliation":[{"name":"William G. Lowrie Department of Chemical and Biomolecular Engineering College of Engineering, The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4698-2332","authenticated-orcid":false,"given":"Mohd Asim","family":"Khan","sequence":"additional","affiliation":[{"name":"William G. Lowrie Department of Chemical and Biomolecular Engineering College of Engineering, The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8794-2839","authenticated-orcid":false,"given":"Pedro","family":"Cabrales","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of California San Diego  La Jolla California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Irwin","sequence":"additional","affiliation":[{"name":"Cardiovascular and Pulmonary Research Group, Department of Medicine University of Colorado, Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0687-3008","authenticated-orcid":false,"given":"Paul W.","family":"Buehler","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine, Center for Blood Oxygen Transport and Hemostasis Department Pediatrics  Baltimore Maryland USA"},{"name":"Department of Pathology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3378-3772","authenticated-orcid":false,"given":"Andre F.","family":"Palmer","sequence":"additional","affiliation":[{"name":"William G. Lowrie Department of Chemical and Biomolecular Engineering College of Engineering, The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200800625"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20102836(84)90342\u20105"},{"issue":"1","key":"e_1_2_10_4_1","first-page":"14","article-title":"Inheritance of haptoglobin subtypes","volume":"14","author":"Smithies O","year":"1962","journal-title":"Am J Hum 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