{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T05:45:14Z","timestamp":1791783914488,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Vero cells (referred to as \u201cVero\u201d henceforth) are a commonly used host for the production of a wide variety of vaccines, but due in part to the typically anchorage\u2010dependent nature of the cells, Vero cells also pose multiple challenges during process development, scale\u2010up, and commercialization. The adaptation of Vero to proliferate in a serum\u2010free suspension culture while remaining non\u2010tumorigenic can be a challenging endeavor, and where successful, has often utilized undisclosed and\/or complex custom\u2010designed medium formulations. Additional and expanded options for commercially available serum\u2010free media that can support the robust expansion of suspension\u2010adapted Vero cells for viral vaccine manufacturing would be a significant advantage for increasing the industrial adoption of suspension Vero, as virus production can also be highly medium\u2010dependent and sensitive. In this work, we have successfully transitioned suspension\u2010adapted Vero cells from a proprietary medium to a commercially\u2010available base medium (ExCell VPRO) with supplementation of additional commercially\u2010available components, and subsequently infected the resulting ExCell VPRO cell bank lineage with an attenuated vaccine strain Mumps virus. Like many suspension cell line media formulations, off\u2010the\u2010shelf commercially available ExCell VPRO medium contains dextran sulfate, which serves to minimize cell clumping, but is also well\u2010known to directly impact the infectivity of some viruses. Unexpectedly, we have observed that the presence of dextran sulfate in the infection medium increased both culture longevity and infectious virus titers for attenuated Mumps, potentially eliminating the need for a medium exchange between cell growth and infection for a more streamlined and easily scalable attenuated Mumps virus production process.<\/jats:p>","DOI":"10.1002\/btpr.88561","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T04:56:42Z","timestamp":1791781002000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adaptation of suspension\n                    <scp>Vero<\/scp>\n                    cells to commercially available cell culture media and enhanced production of vaccine\u2010strain mumps virus"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4047-1436","authenticated-orcid":false,"given":"Rebecca","family":"Hong","sequence":"first","affiliation":[{"name":"Process Research &amp; Development Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2706-8278","authenticated-orcid":false,"given":"Thomas R.","family":"Blanda","sequence":"additional","affiliation":[{"name":"Process Research &amp; Development Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2263-5879","authenticated-orcid":false,"given":"Maria","family":"Bednar","sequence":"additional","affiliation":[{"name":"Process Research &amp; Development Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0765-9954","authenticated-orcid":false,"given":"Jessica","family":"Olson","sequence":"additional","affiliation":[{"name":"Process Research &amp; Development Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise K. B.","family":"Baron","sequence":"additional","affiliation":[{"name":"Global Quality Large Molecule Analytical Sciences (GQLMAS) Merck &amp; Co., Inc.  West Point Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6796-8361","authenticated-orcid":false,"suffix":"III","given":"Daniel","family":"Spatafore","sequence":"additional","affiliation":[{"name":"Process Research &amp; Development Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duygu Ercan","family":"Oruc","sequence":"additional","affiliation":[{"name":"Process Research &amp; Development Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7258-2612","authenticated-orcid":false,"given":"James M.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Process Research &amp; Development Merck &amp; Co., Inc.  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