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A total of 51 cardiac computed tomography scans and 120 adult autopsied human hearts without cardiovascular disease were examined. The 3D reconstruction and visualization software (Mimics Innovation Suite 22, Materialise) was used for heart chambers semi\u2010automatic segmentation and myocardial manual segmentation to visualize a 3D structure of the mitral valve complex and to perform all measurements. Direct comparison of corresponding mitral valve parameters revealed significant differences between obtained results. Significantly larger intercommisural diameter, aorto\u2010mural diameter, and perimeter of the mitral annulus were observed in tomographic scans (all <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). However, the intercommissural\/aorto\u2010mural diameter ratio showed comparable values for both groups. Nevertheless, the size of anterior mitral leaflet was higher in autopsied material. The height of the P2 scallops was the only parameter that show no significant difference between two groups (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.3). The use of 3D postprocessing algorithms provides a very accurate image of the mitral valve structure, which could be useful for the precise non\u2010invasive assessment of mitral valve size and structure. Three\u2010dimensional contrast enhanced cardiac computed tomography significantly overestimates the measurements of the mitral annulus compared to postmortem analysis.<\/jats:p>","DOI":"10.1002\/ca.23967","type":"journal-article","created":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T09:09:25Z","timestamp":1666429765000},"page":"250-255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Three\u2010dimensional cardiac computed tomography compared with autopsied material for the assessment of the mitral valve"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7691-8174","authenticated-orcid":false,"given":"Agata","family":"Krawczyk\u2010O\u017c\u00f3g","sequence":"first","affiliation":[{"name":"Department of Anatomy, HEART \u2013 Heart Embryology and Anatomy Research Team Jagiellonian University Medical College  Krakow Poland"},{"name":"Department of Cardiology and 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