{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T06:59:57Z","timestamp":1773471597929,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T00:00:00Z","timestamp":1762646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T00:00:00Z","timestamp":1762646400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Anatomy"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recently, great attention has been given to understanding the new pathogenetic mechanisms underlying aortic stenosis (\n                    <jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content>\n                    ). The study aims to understand the role of mature adipocytes in\n                    <jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content>\n                    and their association with histologic, clinical, and echocardiographic data, an area previously overlooked in\n                    <jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content>\n                    research. We enrolled 25 patients (15 women and 10 men) with severe\n                    <jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content>\n                    undergoing elective aortic valve replacement. Each patient underwent clinical and transthoracic echocardiographic evaluations before surgery. We obtained\n                    <jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content>\n                    valves and left ventricular (\n                    <jats:styled-content style=\"fixed-case\">LV<\/jats:styled-content>\n                    ) septal biopsies to assess the presence of adipocytes within the valve using perilipin 1 (\n                    <jats:styled-content style=\"fixed-case\">PLIN1<\/jats:styled-content>\n                    ) immunohistochemistry, and we also examined other histological characteristics of the ventricular biopsies. Adipocytes were detected in 76% of the aortic stenotic valve samples, often grouped adjacent to calcified areas. Patients with higher values of PLIN1 valvular adipocytes were generally older (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.06) and had lower BMI values (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.06). Moreover, the group with a higher presence of PLIN1(+) valvular adipocytes had significantly decreased mean gradient values and reduced M1 macrophage infiltration in ventricular biopsies. In a binary regression analysis, only mean gradient was significantly associated with the presence of PLIN1(+) adipocytes in the valve, regardless of age, BMI and ventricular M1 macrophage levels. These preliminary findings suggest that valvular adipocytes could be related to the progression of\n                    <jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content>\n                    , but more investigation is necessary.\n                  <\/jats:p>","DOI":"10.1002\/ca.70045","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T07:15:36Z","timestamp":1762758936000},"page":"325-331","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adipocytes in Aortic Stenosis: Association With Clinical and Morphological Indices"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5235-1545","authenticated-orcid":false,"given":"Elena","family":"Zoico","sequence":"first","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanaz","family":"Saatchi","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Urbani","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanni","family":"Rizzatti","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria","family":"Mazzali","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Fantin","sequence":"additional","affiliation":[{"name":"DIPSCO, Department of Psychology and Cognitive Science CISMed University of Trento  Trento Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Faccioli","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3195-0834","authenticated-orcid":false,"given":"Alessandro","family":"Gavras","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Zamboni","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6864-8653","authenticated-orcid":false,"given":"Anna","family":"Giani","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine University of Verona  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcad.2023.06.002"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcibr2200439"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jebm.12542"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacbts.2023.01.009"},{"key":"e_1_2_10_6_1","first-page":"179","volume-title":"Histology for Pathologists","author":"Brooks J. S. J.","year":"2012"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2023.131155"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ehjci\/ehaa946.3721"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abf7910"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.104.486738"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2016.11.180"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2010.202"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222011081"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.933036"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12074"},{"issue":"11","key":"e_1_2_10_16_1","first-page":"409","article-title":"Pathogenesis and Pathophysiology of Aortic Valve Stenosis in Adults","volume":"121","author":"Olszowska M.","year":"2011","journal-title":"Polskie Archiwum Medycyny Wewn\u0119trznej"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.90.2.844"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2020.107276"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncpcardio0827"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.shj.2022.100089"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242115508"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.22008"}],"container-title":["Clinical Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ca.70045","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ca.70045","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ca.70045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T06:09:29Z","timestamp":1773468569000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ca.70045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,9]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/ca.70045"],"URL":"https:\/\/doi.org\/10.1002\/ca.70045","archive":["Portico"],"relation":{},"ISSN":["0897-3806","1098-2353"],"issn-type":[{"value":"0897-3806","type":"print"},{"value":"1098-2353","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,9]]},"assertion":[{"value":"2025-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}