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Despite being a highly radio\u2010sensitive and treatable cancer, a majority of NPC patients are diagnosed in their advanced stage, and locoregional and distant relapses following definitive treatment contribute largely to cancer\u2010specific mortality among these patients. Given that EBV\u2010driven NPC is the predominant variant seen in endemic regions, various EBV detection methods have been developed and are utilized in screening, prognostication, and post\u2010treatment surveillance of NPC patients. While the Immunoglobulin A (IgA) serology assay is the most extensively studied EBV detection method, the detection of plasma EBV DNA released during replication or cellular apoptosis has shown superior outcomes in endemic population screening, prognostication, and detection of distant relapse. Furthermore, there is emerging evidence on the use of circulating tumor cells, microRNAs, DNA hypermethylation, and combination assays in various clinical scenarios. Herein, this paper provides a comprehensive overview of the relevant studies using various EBV detection techniques in the management of NPC. Specifically, the recent advances, clinical evidence, and challenges associated with the clinical application of EBV liquid biopsies in population screening, prognostication, and surveillance of NPC are presented.<\/jats:p>","DOI":"10.1002\/cac2.12100","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T03:37:21Z","timestamp":1601350641000},"page":"564-585","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Clinical utility of Epstein\u2010Barr virus DNA and other liquid biopsy markers in nasopharyngeal carcinoma"],"prefix":"10.34133","volume":"40","author":[{"given":"Rong","family":"Tan","sequence":"first","affiliation":[{"name":"National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean Kean Ann","family":"Phua","sequence":"additional","affiliation":[{"name":"National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoke Lim","family":"Soong","sequence":"additional","affiliation":[{"name":"National Cancer Center","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynette Lin Ean","family":"Oon","sequence":"additional","affiliation":[{"name":"Singapore General Hospital","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kian Sing","family":"Chan","sequence":"additional","affiliation":[{"name":"Singapore General Hospital","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasidharan Swarnalatha","family":"Lucky","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering and Nanotechnology","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Mong","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering and Nanotechnology","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Han","family":"Tan","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering and Nanotechnology","place":["Singapore, Singapore"]},{"name":"Lucence Diagnostics","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chwee Ming","family":"Lim","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering and Nanotechnology","place":["Singapore, Singapore"]},{"name":"Singapore General Hospital","place":["Singapore, Singapore"]},{"name":"Duke\u2010NUS Graduate Medical School","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"issue":"2","key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"114","DOI":"10.5732\/cjc.010.10377","article-title":"The prevalence and prevention of nasopharyngeal carcinoma in China","volume":"30","author":"Cao S\u2010M","year":"2011","unstructured":"Cao S\u2010M, Simons MJ, Qian C\u2010N. 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