{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:35:10Z","timestamp":1786977310710,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T00:00:00Z","timestamp":1491955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanghai Science and Technology Commission","award":["15140903000"],"award-info":[{"award-number":["15140903000"]}]},{"DOI":"10.13039\/501100014175","name":"Shanghai Municipal Health and Family Planning Commission","doi-asserted-by":"crossref","award":["201540224"],"award-info":[{"award-number":["201540224"]}],"id":[{"id":"10.13039\/501100014175","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Abstract<\/jats:title>\n \n Ovarian cancer with the highest mortality rate among gynecological malignancies is one of common cancers among female cancer patients. As reported in recent years,\n AEG<\/jats:styled-content>\n \u20101 was associated with the occurrence, development, and metastasis of ovarian cancer, but the mechanisms remain unclear. In the current study, invasion capabilities of ovarian cancer\n OVCAR<\/jats:styled-content>\n 3 cells were measured by viral infection and transwell assay. Western blot analysis was used to evaluate the expression levels of\n \u03b2<\/jats:italic>\n \u2010catenin, E\u2010cadherin,\n MMP<\/jats:styled-content>\n 2, and\n MMP<\/jats:styled-content>\n 9. With\n qRT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n analysis,\n AEG<\/jats:styled-content>\n \u20101 and\n HIF<\/jats:styled-content>\n \u20101\n \u03b1<\/jats:italic>\n gene expression were detected. We used luciferase reporter gene to measure\n AEG<\/jats:styled-content>\n \u20101 promoter activity under normoxia\/hypoxia in\n OVCAR<\/jats:styled-content>\n 3 cells. Our work demonstrated that\n AEG<\/jats:styled-content>\n \u20101 significantly enhanced invasion capabilities of\n OVCAR<\/jats:styled-content>\n 3 cells and the expression levels of\n \u03b2<\/jats:italic>\n \u2010catenin, E\u2010cadherin,\n MMP<\/jats:styled-content>\n 2, and\n MMP<\/jats:styled-content>\n 9 associated with invasion capabilities of\n OVCAR<\/jats:styled-content>\n 3 cells were upregulated. Furthermore, hypoxia enhanced invasion capabilities of\n OVCAR<\/jats:styled-content>\n 3 cells and induced\n AEG<\/jats:styled-content>\n \u20101 high gene expression, which was reversed by\n AEG<\/jats:styled-content>\n \u20101 knockdown lentivirus.\n HIF<\/jats:styled-content>\n \u20101\n \u03b1<\/jats:italic>\n expression upregulation was induced in\n OVCAR<\/jats:styled-content>\n 3 cells after hypoxia.\n HIF<\/jats:styled-content>\n \u20101\n \u03b1<\/jats:italic>\n knockdown lentivirus induced downregulated expression of\n AEG<\/jats:styled-content>\n \u20101 and invasion capabilities of\n OVCAR<\/jats:styled-content>\n 3 cells were also inhibited. Wild\u2010type\n AEG<\/jats:styled-content>\n \u20101 promoter activity under hypoxic conditions was significantly higher than that AEG\u20101 mutation under normoxic conditions in the absence of hypoxia response. Our results suggested that\n HIF<\/jats:styled-content>\n \u20101\n \u03b1<\/jats:italic>\n binds to\n AEG<\/jats:styled-content>\n \u20101 promoter to upregulate its expression, which was correlated with metastasis in ovarian cancer by inducing the expression of\n MMP<\/jats:styled-content>\n 2 and\n MMP<\/jats:styled-content>\n 9 as well as inhibiting expression of E\u2010cadherin and\n \u03b2<\/jats:italic>\n \u2010catenin.\n <\/jats:p>","DOI":"10.1002\/cam4.1053","type":"journal-article","created":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T03:24:42Z","timestamp":1491967482000},"page":"1072-1081","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["HIF<\/scp>\n \u20101\n \u03b1<\/i>\n binding to\n AEG<\/scp>\n \u20101 promoter induced upregulated\n AEG<\/scp>\n \u20101 expression associated with metastasis in ovarian cancer"],"prefix":"10.1002","volume":"6","author":[{"given":"Ting","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Gynecology Obstetrics and Gynecology Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology Obstetrics and Gynecology Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy East China University of Science and Technology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Pharmacy East China University of Science and Technology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Gao","sequence":"additional","affiliation":[{"name":"The Diagnosis and Treatment Center of Cervical Disease Obstetrics and Gynecology Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2961-3770","authenticated-orcid":false,"given":"Yuan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Gynecology Obstetrics and Gynecology Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2007.12.015"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-10-0193"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-10-0195"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djt431"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.05.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2011.766"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201101094"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1080\/13550280390201263","article-title":"Identification of gene products suppressed by human immunodeficiency virus type 1 infection or gp120 exposure of primary human astrocytes by rapid subtraction hybridization","volume":"9","author":"Su Z. 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