{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:25:40Z","timestamp":1790839540749,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T00:00:00Z","timestamp":1377561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Epithelial ovarian cancer (\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    ) is the most lethal gynecologic malignancy. Despite initial responsiveness, 80% of\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    patients recur and present with chemoresistant and a more aggressive disease. This suggests an underlying biology that results in a modified recurrent disease, which is distinct from the primary tumor. Unfortunately, the management of recurrent\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    is similar to primary disease and does not parallel the molecular changes that may have occurred during the process of rebuilding the tumor. We describe the characterization of unique in vitro and in vivo ovarian cancer models to study the process of recurrence. The in vitro model consists of\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    +\/\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    44+\/MyD88+\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    stem cells and mCherry+\/\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    44\u2212\/MyD88\u2212\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    cells. The in vivo model consists of mCherry+\/\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    44+\/MyD88+\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    cells injected intraperitoneally. Animals received four doses of Paclitaxel and response to treatment was monitored by in vivo imaging. Phenotype of primary and recurrent disease was characterized by quantitative polymerase chain reaction (\n                    <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>\n                    ) and Western blot analysis. Using the in vivo and in vitro models, we confirmed that chemotherapy enriched for\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    44+\/MyD88+\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    stem cells. However, we observed that the surviving\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    44+\/MyD88+\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    stem cells acquire a more aggressive phenotype characterized by chemoresistance and migratory potential. Our results highlight the mechanisms that may explain the phenotypic heterogeneity of recurrent\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    and emphasize the significant plasticity of ovarian cancer stem cells. The significance of our findings is the possibility of developing new venues to target the surviving\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    44+\/MyD88+\n                    <jats:styled-content style=\"fixed-case\">EOC<\/jats:styled-content>\n                    stem cells as part of maintenance therapy and therefore preventing recurrence and metastasis, which are the main causes of mortality in patients with ovarian cancer.\n                  <\/jats:p>","DOI":"10.1002\/cam4.115","type":"journal-article","created":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T03:49:52Z","timestamp":1377575392000},"page":"751-762","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Phenotypic modifications in ovarian cancer stem cells following Paclitaxel treatment"],"prefix":"10.1002","volume":"2","author":[{"given":"Vinicius","family":"Craveiro","sequence":"first","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang\u2010Hartwich","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennie C.","family":"Holmberg","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia J.","family":"Sumi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Pizzonia","sequence":"additional","affiliation":[{"name":"Bruker Corporation Billerica Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Griffin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina K.","family":"Gill","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan\u2010Arin","family":"Silasi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoud","family":"Azodi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Rutherford","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha B.","family":"Alvero","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gil","family":"Mor","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences Reproductive Immunology Unit Yale University School of Medicine New Haven 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