{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:11:58Z","timestamp":1789683118741,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,11,10]],"date-time":"2017-11-10T00:00:00Z","timestamp":1510272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["17\u201054\u201033009"],"award-info":[{"award-number":["17\u201054\u201033009"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The B\u2010cell receptor (BCR) signaling pathway is of great importance for B\u2010cell survival and proliferation. The BCR expressed on malignant B\u2010CLL cells contributes to the disease pathogenesis, and its signaling pathway is currently the target of several therapeutic strategies. Although various BCR alterations have been described in B\u2010CLL at the protein level, the mRNA expression levels of tyrosine kinases in B\u2010CLL compared to that in normal CD5\u2010high and CD5\u2010low B\u2010lymphocytes remain unknown. In the current study, we measured the mRNA expression levels of<jats:italic>CD79A<\/jats:italic>,<jats:italic>CD79B<\/jats:italic>,<jats:italic>LYN<\/jats:italic>,<jats:italic>SYK<\/jats:italic>,<jats:italic>SHP1<\/jats:italic>, and<jats:italic>ZAP70<\/jats:italic>in purified populations of CD5\u2010high B\u2010CLL cells, CD5\u2010low B\u2010cells from the peripheral blood of healthy donors, and CD5\u2010high B\u2010cells from human tonsils. Here, we report a clear separation in the B\u2010CLL dataset between the<jats:italic>ZAP70<\/jats:italic>\u2010high and<jats:italic>ZAP70<\/jats:italic>\u2010low subgroups. Each subgroup has a unique expression profile of BCR signaling components that might reflect the functional status of the BCR signaling pathway. Moreover, the<jats:italic>ZAP70\u2010<\/jats:italic>low subgroup does not resemble either CD5\u2010high B\u2010lymphocytes from the tonsils or CD5\u2010low lymphocytes from PBMC (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). We also show that<jats:italic>ZAP70<\/jats:italic>is the only gene that is differentially expressed in CD5\u2010high and CD5\u2010low normal B\u2010lymphocytes, confirming the key role of Zap\u201070 tyrosine kinase in BCR signaling alterations in B\u2010CLL.<\/jats:p>","DOI":"10.1002\/cam4.1257","type":"journal-article","created":{"date-parts":[[2017,11,10]],"date-time":"2017-11-10T11:42:47Z","timestamp":1510314167000},"page":"2984-2997","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of the<scp>mRNA<\/scp>expression profile of B\u2010cell receptor components in normal<scp>CD<\/scp>5\u2010high B\u2010lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3294-7146","authenticated-orcid":false,"given":"Aleena A.","family":"Gladkikh","sequence":"first","affiliation":[{"name":"Biological Department M.V. 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Lomonosov Moscow State University Moscow Russia"},{"name":"N.N. Blokhin Russian Cancer Research Center Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha","family":"Barteneva","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Harvard Medical School Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Vorobjev","sequence":"additional","affiliation":[{"name":"Biological Department M.V. Lomonosov Moscow State University Moscow Russia"},{"name":"Department of Biology School of Science and Technology Nazarbayev University Astana Kazakhstan"},{"name":"A.N. Belozersky Institute of Physico\u2010Chemical Biology M.V. Lomonosov Moscow State University Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1586\/era.10.127"},{"key":"e_1_2_7_3_1","first-page":"347","article-title":"Interaction of Epstein\u2010Barr virus with leukaemic B cells in vitro. I. Abortive infection and rare cell line establishment from chronic lymphocytic leukaemic cells","volume":"50","author":"Rickinson A. B.","year":"1982","journal-title":"Clin. Exp. 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