{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T08:53:26Z","timestamp":1764060806827,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T00:00:00Z","timestamp":1519344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteosarcoma is one of the most common primary bone tumors in childhood and adolescence. Metastases occurrence at diagnosis or during disease evolution is the main therapeutic challenge. New drug evaluation to improve patient survival requires the development of various preclinical models mimicking at best the complexity of the disease and its metastatic potential. We describe here the development and characteristics of two orthotopic bioluminescent (Luc\/<jats:styled-content style=\"fixed-case\">mK<\/jats:styled-content>ate2) cell\u2010derived xenograft (<jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content>) models, Saos\u20102\u2010B\u2010Luc\/<jats:styled-content style=\"fixed-case\">mK<\/jats:styled-content>ate2\u2010<jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HOS<\/jats:styled-content>\u2010Luc\/<jats:styled-content style=\"fixed-case\">mK<\/jats:styled-content>ate2\u2010<jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content>, in different immune (nude and <jats:styled-content style=\"fixed-case\">NSG<\/jats:styled-content> mouse strains) and bone (intratibial and paratibial with periosteum activation) contexts. <jats:styled-content style=\"fixed-case\">IVIS<\/jats:styled-content> Spectrum<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> system allowed both longitudinal computed tomography (<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>) and bioluminescence real\u2010time follow\u2010up of primary tumor growth and metastatic spread, which was confirmed by histology. The murine immune context influenced tumor engraftment, primary tumor growth, and metastatic spread to lungs, bone, and spleen (an unusual localization in humans). Engraftment in <jats:styled-content style=\"fixed-case\">NSG<\/jats:styled-content> mice was found superior to that found in nude mice and intratibial bone environment more favorable to engraftment compared to paratibial injection. The genetic background of the two <jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content> models also led to distinct primary tumor behavior observed on <jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> scan. Saos\u20102\u2010B\u2010Luc\/<jats:styled-content style=\"fixed-case\">mK<\/jats:styled-content>ate2\u2010<jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content> showed osteocondensed, <jats:styled-content style=\"fixed-case\">HOS<\/jats:styled-content>\u2010Luc\/<jats:styled-content style=\"fixed-case\">mK<\/jats:styled-content>ate2\u2010<jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content> osteolytic morphology. Bioluminescence defined a faster growth of the primary tumor and metastases in Saos\u20102\u2010B\u2010Luc\/<jats:styled-content style=\"fixed-case\">mK<\/jats:styled-content>ate2\u2010<jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content> than in <jats:styled-content style=\"fixed-case\">HOS<\/jats:styled-content>\u2010Luc\/<jats:styled-content style=\"fixed-case\">mK<\/jats:styled-content>ate2\u2010<jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content>. The early detection of primary tumor growth and metastatic spread by bioluminescence allows an improved exploration of osteosarcoma disease at tumor progression, and metastatic spread, as well as the evaluations of anticancer treatments. Our orthotopic models with metastatic spread bring complementary information to other types of existing osteosarcoma models.<\/jats:p>","DOI":"10.1002\/cam4.1346","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T07:18:40Z","timestamp":1519370320000},"page":"665-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Establishment and characterization of <i>in vivo<\/i> orthotopic bioluminescent xenograft models from human osteosarcoma cell lines in Swiss nude and <scp>NSG<\/scp> mice"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1498-603X","authenticated-orcid":false,"given":"Maria Eugenia","family":"Marques da Costa","sequence":"first","affiliation":[{"name":"Vectorology and Anticancer Therapies UMR 8203, CNRS University of Paris\u2010Sud Gustave Roussy, Universit\u00e9 Paris\u2010Saclay  Villejuif France"},{"name":"CESAM &amp; Department of Biology University of Aveiro  Aveiro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5065-5679","authenticated-orcid":false,"given":"Estelle","family":"Daudigeos\u2010Dubus","sequence":"additional","affiliation":[{"name":"Vectorology and Anticancer Therapies UMR 8203, CNRS University of Paris\u2010Sud Gustave Roussy, Universit\u00e9 Paris\u2010Saclay  Villejuif France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1223-8393","authenticated-orcid":false,"given":"Anne","family":"Gomez\u2010Brouchet","sequence":"additional","affiliation":[{"name":"Department of Pathology IUCT\u2010Oncopole CHU of Toulouse and University of Toulouse  Toulouse France"},{"name":"Pharmacology and Structural Biology Institut CNRS UMR5089  Toulouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8303-1712","authenticated-orcid":false,"given":"Olivia","family":"Bawa","sequence":"additional","affiliation":[{"name":"Plateforme HistoCytoPathologie UMS AMMICa, Gustave Roussy  Villejuif France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8022-3081","authenticated-orcid":false,"given":"Valerie","family":"Rouffiac","sequence":"additional","affiliation":[{"name":"Imaging and Cytometry Platform UMS 3655&amp; US23 Gustave Roussy, Paris\u2010Saclay University  Villejuif France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0742-1177","authenticated-orcid":false,"given":"Massimo","family":"Serra","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Oncology Orthopaedic Rizzoli Institute  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6114-9499","authenticated-orcid":false,"given":"Katia","family":"Scotlandi","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Oncology Orthopaedic Rizzoli Institute  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4129-6381","authenticated-orcid":false,"given":"Concei\u00e7\u00e3o","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Sciences University of Porto  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4361-3643","authenticated-orcid":false,"given":"Birgit","family":"Geoerger","sequence":"additional","affiliation":[{"name":"Vectorology and Anticancer Therapies UMR 8203, CNRS University of Paris\u2010Sud Gustave Roussy, Universit\u00e9 Paris\u2010Saclay  Villejuif France"},{"name":"Department of Oncologie for child and adolescent Gustave Roussy  Villejuif France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1827-8903","authenticated-orcid":false,"given":"Nathalie","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Vectorology and Anticancer Therapies UMR 8203, CNRS University of Paris\u2010Sud Gustave Roussy, Universit\u00e9 Paris\u2010Saclay  Villejuif France"},{"name":"Department of Oncologie for child and adolescent Gustave Roussy  Villejuif France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(16)00162-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.60.0734"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(16)30096-1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq276"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.43.8598"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.59.4895"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2017.01.005"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/627254"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2011.72"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090727"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20717"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048262"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbo.2012.12.005"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085128"},{"key":"e_1_2_8_16_1","first-page":"2333","article-title":"Bone microenvironment has an influence on the histological response of osteosarcoma to chemotherapy: retrospective analysis and preclinical modeling","volume":"7","author":"Crenn V.","year":"2017","journal-title":"Am. 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