{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:39:49Z","timestamp":1784864389142,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:00:00Z","timestamp":1540339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81602571"],"award-info":[{"award-number":["81602571"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2018,11]]},"abstract":"Abstract<\/jats:title>14\u20103\u20103\u03b6<\/jats:italic>, a phosphopeptide\u2010binding molecule, is reportedly overexpressed in the cancerous tissues of patients with hepatocellular carcinoma (HCC<\/jats:styled-content>). Hepatitis B virus (HBV<\/jats:styled-content>) protein X (HB<\/jats:styled-content>x) draws intensive attention in HBV<\/jats:styled-content>\u2010related HCC<\/jats:styled-content> because it not only regulates HBV<\/jats:styled-content> replication, but also promotes carcinogenesis by interacting with various tumor or antitumor molecules. This study is performed to investigate whether and how 14\u20103\u20103\u03b6<\/jats:italic> interacts with HB<\/jats:styled-content>x. The coimmunoprecipitation (Co\u2010IP<\/jats:styled-content>) results showed that 14\u20103\u20103\u03b6<\/jats:italic> bond to HB<\/jats:styled-content>x in HBV<\/jats:styled-content>\u2010infected Hep3B HCC<\/jats:styled-content> cells and CSQT<\/jats:styled-content>\u20102 portal vein tumor thrombosis (PVTT<\/jats:styled-content>) cells. By performing Co\u2010IP<\/jats:styled-content> assay in HBV<\/jats:styled-content>\u2010free Huh7 cells expressing wild\u2010type HB<\/jats:styled-content>x, mutant HB<\/jats:styled-content>x\u2010S31A, or HB<\/jats:styled-content>x\u2010S31D (serine31<\/jats:sup> was mutated into alanine31<\/jats:sup> or aspartic acid31<\/jats:sup>), we found that the phosphorylated serine31<\/jats:sup> with its near amino acid residues constituted a RPL<\/jats:styled-content>phosphoS31<\/jats:sup>GP<\/jats:styled-content> (R, arginine; P, proline; L, leucine; S, serine; G, glycine) motif in HB<\/jats:styled-content>x for 14\u20103\u20103\u03b6<\/jats:italic> docking. This 14\u20103\u20103\u03b6<\/jats:italic>\u2010HB<\/jats:styled-content>x interaction was partly impaired when Akt signaling transduction was blocked by LY<\/jats:styled-content>294002. Furthermore, 14\u20103\u20103\u03b6<\/jats:italic> silencing augmented HB<\/jats:styled-content>x ubiquitination and decreased its expression in cancer cells and xenograft tumor. The migratory and invasive abilities of CSQT<\/jats:styled-content>\u20102 cells were inhibited upon 14\u20103\u20103\u03b6<\/jats:italic> silencing, whereas partly restored by HB<\/jats:styled-content>x overexpression. Additionally, 14\u20103\u20103\u03b6<\/jats:italic> positively correlated with HB<\/jats:styled-content>x to be overexpressed in the primary HCC<\/jats:styled-content> tissues (r<\/jats:italic>\u00a0=\u00a00.344) and metastatic PVTT<\/jats:styled-content> (r<\/jats:italic>\u00a0=\u00a00.348). In summary, findings of this study reveal a novel 14\u20103\u20103\u03b6<\/jats:italic>\u2010HB<\/jats:styled-content>x interaction in HCC<\/jats:styled-content> cells and suggest 14\u20103\u20103\u03b6<\/jats:italic> as a candidate target for treating HBV<\/jats:styled-content>\u2010related HCC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/cam4.1512","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T04:08:56Z","timestamp":1540440536000},"page":"5543-5553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["14\u20103\u20103\u03b6<\/i> binds to hepatitis B virus protein X and maintains its protein stability in hepatocellular carcinoma cells"],"prefix":"10.1002","volume":"7","author":[{"given":"Yufu","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery The General Hospital of Shenyang Military Area Command Shenyang 100016 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