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Cancer\u2010associated fibroblasts play key roles in tumor growth and invasiveness and have been regarded as a potential therapeutic target. This study was designed to isolate human primary cancer\u2010associated fibroblasts of intrahepatic cholangiocarcinoma to study tumor\u2010stroma interactions and to analyze the clinical relevance of alpha\u2010smooth muscle actin \u2010positive cancer\u2010associated fibroblasts in patients with intrahepatic cholangiocarcinoma. The isolated cancer\u2010associated fibroblasts were positive for alpha\u2010smooth actin, fibroblast\u2010specific protein\u20101, fibroblast activation protein, and\n                    <jats:styled-content style=\"fixed-case\">PDGFR<\/jats:styled-content>\n                    \u2010\u03b2. In addition, cancer\u2010associated fibroblasts were found to increase proliferation, migration, and invasion of cholangiocarcinoma cells in vitro and promote tumor growth of mice in vivo. Moreover, alpha\u2010smooth muscle actin\u2010positive expression of cancer\u2010associated fibroblasts predicted unfavorable prognosis in patients with intrahepatic cholangiocarcinoma and showed correlation with presence of lymph node metastasis. This study may provide a useful tool to investigate further effect of cancer\u2010associated fibroblasts on the molecular mechanism of cholangiocarcinoma cells as well as contribution of cancer\u2010associated fibroblasts in lymphangiogenesis and lymph node metastasis.\n                  <\/jats:p>","DOI":"10.1002\/cam4.1704","type":"journal-article","created":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T03:55:11Z","timestamp":1533009311000},"page":"4665-4677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Isolation of cancer\u2010associated fibroblasts and its promotion to the progression of intrahepatic cholangiocarcinoma"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4492-1189","authenticated-orcid":false,"given":"Meng","family":"Sha","sequence":"first","affiliation":[{"name":"Department of Liver Surgery Ren Ji Hospital School of Medicine Shanghai Jiao Tong 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