{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T06:08:24Z","timestamp":1784268504381,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,11,8]],"date-time":"2018-11-08T00:00:00Z","timestamp":1541635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572976"],"award-info":[{"award-number":["81572976"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016M590505"],"award-info":[{"award-number":["2016M590505"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2017T100407"],"award-info":[{"award-number":["2017T100407"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Temozolomide (TMZ) is widely used to treat melanoma; however, response rates to TMZ are low because of rapid and frequent resistance. Conditionally, replicative adenoviruses (CRAds) are an effective and promising approach. The receptor for adenovirus is coxsackie\u2010adenovirus receptor (CAR), which is poorly expressed in most cells. However, CD46, which is the receptor of species B adenoviruses (Ads), is highly expressed in many cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We constructed CRAd F5\/35\u2010ZD55\u2010IL\u201024, which uses the viral receptors CAR and CD46 for entry into cells. We investigated the antitumor effect of F5\/35\u2010ZD55\u2010IL\u201024 in combination with TMZ to treat melanoma in vitro and in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The \\results indicated that F5\/35\u2010ZD55\u2010IL\u201024 in combination with TMZ produced additive or synergistic antitumor and pro\u2010apoptotic effects in melanoma cells. The combination of F5\/35\u2010ZD55\u2010IL\u201024 and TMZ significantly inhibited the growth of melanoma in vivo. In addition, the antitumor effect of F5\/35\u2010ZD55\u2010IL\u201024 was superior to that of ZD55\u2010IL\u201024 and ZD55\u2010IL\u201024 combined with TMZ.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The use of F5\/35\u2010ZD55\u2010IL\u201024 in conjunction with TMZ is a promising approach for anti\u2010melanoma therapy.<\/jats:p><jats:p>Our results indicated that F5\/35\u2010ZD55\u2010IL\u201024 in combination with TMZ produced additive or synergistic antitumor effect and pro\u2010apoptotic effect in melanoma cells highly expressed CD46. The combination of F5\/35\u2010ZD55\u2010IL\u201024 and TMZ significantly inhibited the growth of melanoma in vivo. We also found the antitumor effect of F5\/35\u2010ZD55\u2010IL\u201024 was superior to ZD55\u2010IL\u201024, the combination of F5\/35\u2010ZD55\u2010IL\u201024 and TMZ had a more significant antitumor effect than ZD55\u2010IL\u201024 combining with TMZ.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.1843","type":"journal-article","created":{"date-parts":[[2018,11,8]],"date-time":"2018-11-08T13:44:56Z","timestamp":1541684696000},"page":"5928-5942","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Combination therapy with F5\/35 fiber chimeric conditionally replicative adenoviruses expressing IL\u201024 enhances the antitumor effect of temozolomide against melanoma"],"prefix":"10.1002","volume":"7","author":[{"given":"Ming","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Dermatology Affiliated Hospital of Xuzhou Medical University Xuzhou China"},{"name":"Department of Radiotherapy Affiliated 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