{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T21:36:25Z","timestamp":1793568985141,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,11]],"date-time":"2019-01-11T00:00:00Z","timestamp":1547164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005624","name":"Medizinischen Hochschule Hannover","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005624","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Atypical chronic myeloid leukemia (aCML) and chronic myelomonocytic leukemia (CMML) represent two histologically and clinically overlapping myelodysplastic\/myeloproliferative neoplasms. Also the mutational landscapes of both entities show congruencies. We analyzed and compared an aCML cohort (n\u00a0=\u00a026) and a CMML cohort (n\u00a0=\u00a059) by next\u2010generation sequencing of 25 genes and by an nCounter approach for differential expression in 107 genes. Significant differences were found with regard to the mutation frequency of <jats:italic>TET2<\/jats:italic>, <jats:italic>SETBP1<\/jats:italic>, and <jats:italic>CSF3R<\/jats:italic>. Blast content of the bone marrow revealed an inverse correlation with the mutation status of <jats:italic>SETBP1<\/jats:italic> in aCML and <jats:italic>TET2<\/jats:italic> in CMML, respectively. By linear discriminant analysis, a mutation\u2010based machine learning algorithm was generated which placed 19\/26 aCML cases (73%) and 54\/59 (92%) CMML cases into the correct category. After multiple correction, differential mRNA expression could be detected between both cohorts in a subset of genes (<jats:italic>FLT3<\/jats:italic>, <jats:italic>CSF3R<\/jats:italic>, and <jats:italic>SETBP1<\/jats:italic> showed the strongest correlation). However, due to high variances in the mRNA expression, the potential utility for the clinic is limited. We conclude that a medium\u2010sized NGS panel provides a valuable assistance for the correct classification of aCML and CMML.<\/jats:p>","DOI":"10.1002\/cam4.1946","type":"journal-article","created":{"date-parts":[[2019,1,12]],"date-time":"2019-01-12T05:11:51Z","timestamp":1547269911000},"page":"742-750","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Comprehensive mutation profiling and mRNA expression analysis in atypical chronic myeloid leukemia in comparison with chronic myelomonocytic leukemia"],"prefix":"10.1002","volume":"8","author":[{"given":"Muhammad","family":"Faisal","sequence":"first","affiliation":[{"name":"Institute of Pathology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helge","family":"Stark","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guntram","family":"B\u00fcsche","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome","family":"Schlue","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Teiken","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans H.","family":"Kreipe","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover 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