{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T22:38:56Z","timestamp":1782254336280,"version":"3.54.5"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T00:00:00Z","timestamp":1549584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Malignant peripheral nerve sheath tumors (MPNSTs) are rare and aggressive soft tissue sarcomas with a significant susceptibility to metastasize early in their course. Pathogenesis is yet to be fully elucidated. Recently, the essential role of mast cells in the tumor onset of neurofibromatosis type 1 (NF1)\u2010associated neurofibromas and MPNSTs was confirmed in both experimental and human studies. In this study, we investigate mast cell density (MCD), microvascular density (MVD), and proliferation index (Ki\u201067) in MPNST. A secondary aim was to correlate histological staining to clinical data and survival in patients with and without NF1. In total, 34 formalin\u2010fixed paraffin\u2010embedded MPNST tissues from 29 patients were eligible. MCD, MVD, and Ki\u201067 labeling index (LI) were analyzed in all stained tissues by a computer\u2010based quantitative algorithm (Aperio ImageScope). In addition, chart review was performed for clinical data and survival analysis. Overall, MCD, MVD, and Ki\u201067 LI were evenly distributed throughout tumor tissue. There was a negative correlation of NF1 status (affected, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.037), tumor size (&gt;10\u00a0cm, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.023), and MVD in the tumor periphery (higher tercile, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.002) to survival. Multivariate analysis confirmed the association of MVD in the tumor periphery (higher tercile, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.019) with a decreased overall survival. Diverse mast cell and microvascular distributions suggest that angiogenesis in MPNST occurs independently. The role of mast cells in tumor progression is unclear and lacks prognostic value. Higher MVD has prognostic significance with possible therapeutic implications in MPNST.<\/jats:p>","DOI":"10.1002\/cam4.1977","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T10:55:10Z","timestamp":1549623310000},"page":"972-981","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Prognostic significance of mast cell and microvascular densities in malignant peripheral nerve sheath tumor with and without neurofibromatosis type 1"],"prefix":"10.1002","volume":"8","author":[{"given":"Roberto Andr\u00e9 Torres de","family":"Vasconcelos","sequence":"first","affiliation":[{"name":"Division of Bone and Connective Tissue, Department of Surgical Oncology National Cancer Institute Rio de Janeiro Brazil"},{"name":"Postgraduation Program in Neurology Federal University of the State of Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Guimar\u00e3es Coscarelli","sequence":"additional","affiliation":[{"name":"Division of Internal Medicine State University of Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thyago Marsicano","family":"Vieira","sequence":"additional","affiliation":[{"name":"Division of Pathology National Cancer Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Washington Silva","family":"Noguera","sequence":"additional","affiliation":[{"name":"Division of Pathology National Cancer Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davy Carlos Mendes","family":"Rapozo","sequence":"additional","affiliation":[{"name":"Division of Pathology National Cancer Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3998-2171","authenticated-orcid":false,"given":"Marcus Andr\u00e9","family":"Acioly","sequence":"additional","affiliation":[{"name":"Postgraduation Program in Neurology Federal University of the State of Rio de Janeiro Rio de Janeiro Brazil"},{"name":"Division of Neurosurgery Federal University of Rio de Janeiro Rio de Janeiro Brazil"},{"name":"Division of Neurosurgery Fluminense Federal University Niter\u00f3i, Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1996.14.5.1679"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-011-1978-7"},{"issue":"6","key":"e_1_2_6_4_1","first-page":"2597","article-title":"Incidence and survival in sarcoma in the United States: a focus on musculoskeletal lesions","volume":"33","author":"Ng VY","year":"2013","journal-title":"Anticancer Res"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22098"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181a77e9a"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-012-2573-2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-30654-4_22"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nos287"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2011.113"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19991008)83:2<171::AID-IJC5>3.0.CO;2-S"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2004.tb00062.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1215\/15228517-2009-003"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nos124"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-8-127"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.suronc.2013.06.003"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-13-1934"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-016-0194-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-13-S1-O6"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms150713060"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2014-0328"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000444942"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.12703\/P7-09"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2009.02.001"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2014.11.014"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20000615)88:12<2686:AID-CNCR25>3.0.CO;2-6"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155414545334"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800094"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-13-0119"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0512245"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI22089"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.5.2586"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-147033"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200319195"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155411407340"},{"issue":"9","key":"e_1_2_6_36_1","first-page":"4713","article-title":"Vessel and mast cell densities in sporadic and syndrome\u2010associated peripheral nerve sheath tumors","volume":"35","author":"Friedrich RE","year":"2015","journal-title":"Anticancer Res"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-2643"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1590\/0004-282x20170052"},{"issue":"3","key":"e_1_2_6_39_1","first-page":"172","article-title":"National Institutes of Health Consensus Development Conference Statement: neurofibromatosis. Bethesda, Md., USA, July 13\u201315, 1987","volume":"1","year":"1988","journal-title":"Neurofibromatosis"},{"key":"e_1_2_6_40_1","volume-title":"Joint Committee on Cancer. AJCC Cancer Staging Manual","author":"Edge SB","year":"2010"},{"issue":"2","key":"e_1_2_6_41_1","first-page":"129","article-title":"Cutaneous manifestation of neurofibromatosis: cellular interaction, pigmentation, and mast cells","volume":"17","author":"Riccardi VM","year":"1981","journal-title":"Birth Defects Orig Artic Ser"},{"issue":"10","key":"e_1_2_6_42_1","first-page":"996","article-title":"Possible involvement of c\u2010kit receptor and its ligand in increase of mast cells in neurofibroma tissues","volume":"117","author":"Hirota S","year":"1993","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.23722"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/730246"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2001.01176.x"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1215\/15228517-2008-127"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2990.2002.00367.x"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2014.10.002"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.pcan.4500572"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2007.05009.x"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcam4.1977","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.1977","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.1977","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.1977","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T08:54:55Z","timestamp":1740214495000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.1977"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,8]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/cam4.1977"],"URL":"https:\/\/doi.org\/10.1002\/cam4.1977","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,8]]},"assertion":[{"value":"2018-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}