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In this study, high expression of NORAD was firstly observed in melanoma tissues and human malignant melanoma cell lines, our aim was to study the interaction of them in the process of invasion and migration of malignant melanoma cells. NORAD, miR\u2010205, and EGLN2 mRNA level in MM cells was detected by qRT\u2010PCR. In situ hybridization (ISH) was performed to detect NORAD expression in MM tissues specimens. Effects of NORAD and miR\u2010205 on Prolyl hydroxylase 2 (EGLN2) expression was explored by western blot in MM cells line. Dual\u2010luciferase reporter assay was performed to verify the interaction relationship between NORAD and miR\u2010205, as well as, miR\u2010205 and EGLN2. Transwell assay was conducted to explore the effects of NORAD and miR\u2010205 in vitro. Xenografts in nude mice experiment were used to confirm the role of NORAD and miR\u2010205 in vivo. In vitro, NORAD knockdown significantly inhibited migration and invasion of malignant melanoma cells and elevated the expression of miR\u2010205, there was an interaction between miR\u2010205 and NORAD in the RNA\u2010induced silencing complex. Upregulation of miR\u2010205 induced significant inhibition of migratory and invasive ability compared with the scrambled control. However, downregulating NORAD largely reversed this effect. Furthermore, the regulatory effects of miR\u2010205 on EGLN2 levels and the induction of endoplasmic reticulum stress were reversed by NORAD. In vivo, deletion of miR\u2010205 induced tumor growth in nude mice. NORAD may play critical roles in tumorigenesis and progression of malignant melanoma by regulating of the miR\u2010205\u2010EGLN2 pathway, and may serve as a new therapeutic target.<\/jats:p>","DOI":"10.1002\/cam4.2046","type":"journal-article","created":{"date-parts":[[2019,3,7]],"date-time":"2019-03-07T13:55:37Z","timestamp":1551966937000},"page":"1744-1754","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Overexpression of long non\u2010coding RNA NORAD promotes invasion and migration in malignant melanoma via regulating the MIR\u2010205\u2010EGLN2 pathway"],"prefix":"10.1002","volume":"8","author":[{"given":"Yong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Plastic Surgery of Third Xiangya Hospital Changsha China"},{"name":"Surgical Department Emergency Department The First Hospital of Changsha Changsha 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