{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T04:05:59Z","timestamp":1771560359545,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T00:00:00Z","timestamp":1558396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["HHSN261201500011C"],"award-info":[{"award-number":["HHSN261201500011C"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R44CA140047"],"award-info":[{"award-number":["R44CA140047"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Circulating tumor cells (CTCs) are important clinical indicators of metastatic progression and treatment efficacy. However, because of their low number and heterogeneity, reliable patient\u2010derived CTC models are not readily available. We report here the isolation and characterization of the invasive population of CTCs, iCTCs, from blood of 10 patients with epithelial ovarian cancer (EOC) and one pancreatic cancer patient based on the avidity of tumor cells toward an artificial collagen\u2010based adhesion matrix (CAM), in comparison with tumor progenitor (TP) cells isolated from tumor cell lines, tumors and ascites from EOC patients. CAM\u2010avid cells identified to be iCTCs were indistinguishable with TP cells using either functional CAM uptake or surface markers (seprase and CD44). In addition, iCTCs were characterized using peritoneal and spontaneous metastasis models in vivo to evaluate their metastatic propensity and therapeutic response. TP cells and iCTCs had a doubling time of about 34\u201042\u00a0hours. TP cells were rare (&lt;3.5%) in most patient\u2010derived specimens, however, iCTCs emigrated into blood, at a high frequency, 64.2% (n\u00a0=\u00a049). Approximately 500 patient\u2010derived iCTCs recapitulated formation of iCTCs in mouse blood and formed micrometastases in the liver and\/or lung, a degree of metastatic spread equivalent to the inoculation of 5\u00a0\u00d7\u00a010<jats:sup>5<\/jats:sup> bulk tumor cells isolated from ascites and tumors. iCTCs were shown to be novel therapeutic targets for blocking metastasis using the reduced formation of iCTCs and micrometastases by RNAi, peptides, and monoclonal antibodies against seprase.<\/jats:p>","DOI":"10.1002\/cam4.2218","type":"journal-article","created":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T05:41:16Z","timestamp":1558503676000},"page":"3864-3874","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The propensity of invasive circulating tumor cells (iCTCs) in metastatic progression and therapeutic responsiveness"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1089-6053","authenticated-orcid":false,"given":"Huan","family":"Dong","sequence":"first","affiliation":[{"name":"Stony Brook Medicine Stony Brook New York"},{"name":"Vitatex Inc Stony Brook New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaun","family":"Tulley","sequence":"additional","affiliation":[{"name":"Stony Brook Medicine Stony Brook New York"},{"name":"Vitatex Inc Stony Brook New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Stony Brook Medicine Stony Brook New York"},{"name":"Vitatex Inc Stony Brook New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leong","family":"Cho","sequence":"additional","affiliation":[{"name":"Stony Brook Medicine Stony Brook New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donghai","family":"Chen","sequence":"additional","affiliation":[{"name":"Stony Brook Medicine Stony Brook New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L.","family":"Pearl","sequence":"additional","affiliation":[{"name":"Stony Brook Medicine Stony Brook New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Tien","family":"Chen","sequence":"additional","affiliation":[{"name":"Stony Brook Medicine Stony Brook New York"},{"name":"Vitatex Inc Stony Brook New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24814"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1379"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-3931"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1253533"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2576"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1600274"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-0145"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34365-z"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers10120467"},{"key":"e_1_2_7_11_1","article-title":"Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. 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