{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:32:12Z","timestamp":1786977132414,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T00:00:00Z","timestamp":1565049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Projects of Mudanjiang City","award":["Z2016s0095"],"award-info":[{"award-number":["Z2016s0095"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bone metastasis of breast cancer makes patients suffer from pain, fractures, spinal cord compression, and hypercalcemia, and is almost incurable. Although the mechanisms of bone metastasis in breast cancers have been studied intensively, novel specific target will be helpful to the development of new therapeutic strategy of breast cancer. Herein, we focused on the microRNA of tumor cell\u2010derived exosomes to investigate the communication between the bone microenvironment and tumor cells. The expression of miR\u201020a\u20105p in the primary murine bone marrow macrophages (BMMs), MCF\u201010A, MCF\u20107, and MDA\u2010MB\u2010231 cell lines, as well as the cell\u2010derived exosomes were assessed by qRT\u2010PCR. Transwell assays were used to evaluate the effects of miR\u201020a\u20105p on tumor cell migration and invasion. The expression of exosomes marker including CD63and TSG101 was detected by Western Blot. Cell cycle distribution of BMMs was analyzed by flow cytometry. 3\u2010UTR luciferase reporter assays were used to validate the putative binding between miR\u201020a\u20105p and SRCIN1. MiR\u201020a\u20105p was highly expressed in breast tumor tissues and the exosomes of MDA\u2010MB\u2010231 cells. MiR\u201020a\u20105p promoted migration and invasion in MDA\u2010MB\u2010231 cells, and the proliferation and differentiation of osteoclasts. MDA\u2010MB\u2010231 cell\u2010derived exosomes transferred miR\u201020a\u20105p to BMMs and facilitated the osteoclastogenesis via targeting SRCIN1. The present work provides evidence that miR\u201020a\u20105p transferred from breast cancer cell\u2010derived exosomes promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1, providing scientific foundations for the development of exosome or miR\u201020a\u20105p targeted therapeutic intervention in breast cancer progression.<\/jats:p>","DOI":"10.1002\/cam4.2454","type":"journal-article","created":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T03:13:35Z","timestamp":1565061215000},"page":"5687-5701","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":100,"title":["Breast cancer cell\u2010derived exosomal miR\u201020a\u20105p promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1"],"prefix":"10.1002","volume":"8","author":[{"given":"Ling","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Pathology The Affiliated Second Hospital Mudanjiang Medical University Mudanjiang Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology Peking University People's Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liandi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Affiliated Second Hospital Mudanjiang Medical University Mudanjiang Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shufen","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Gerontology The Affiliated Second Hospital Mudanjiang Medical University Mudanjiang Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Nursing The Affiliated Second Hospital Mudanjiang Medical University Mudanjiang Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging Mudanjiang Medical University Mudanjiang Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6479-7309","authenticated-orcid":false,"given":"Hujun","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Oncology The Affiliated Hongqi Hospital, Mudanjiang Medical University Mudanjiang Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21320"},{"key":"e_1_2_9_3_1","volume-title":"Breast Cancer Facts & Figures 2017\u20132018","author":"American Cancer 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