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In this study, we isolated and characterized spontaneously immortalized B\u2010lymphocyte (SIBC) lines from HIV\u2010infected patients with and without Non\u2010Hodgkin's Lymphoma (AIDS\u2010NHL). A total of 22 SIBC lines was isolated from peripheral blood mononuclear cells (PBMC) of HIV\u2010infected patients with (n\u00a0=\u00a040) and without (n\u00a0=\u00a077) clinically detectable NHL, but not from healthy individuals (n\u00a0=\u00a034). Of these, 8 SIBC lines named HIV\u2010SIBC were generated from HIV\u2010infected patients without AIDS\u2010NHL (10%, 8\/77), while 14 SIBCs named AIDS\u2010NHL\u2010SIBC were from 13 of the AIDS\u2010NHL patients (32.5%, 13\/40). Among the 14 AIDS\u2010NHL\u2010SIBCs, 12 were derived from AIDS\u2010NHL patients with poor prognoses (survival time less than 1\u00a0year). SIBCs displayed markers typical of memory B cells (CD3<jats:sup>\u2010<\/jats:sup>CD20<jats:sup>+<\/jats:sup>CD27<jats:sup>+<\/jats:sup>) with EBV infection. Moreover, AIDS\u2010NHL\u2010SIBCs were representative of CTC as evidenced by monoclonal Ig gene rearrangement, abnormal chromosomal karyotype, and the formation of xenograft tumors, while HIV\u2010SIBCs generated harbored some features of tumor cells, none had the capacity of xenograft tumor formation, suggesting HIV\u2010SIBC present the precursor of CTC. These results indicate that SIBCs is associated with poor prognosis in AIDS\u2010NHL patients and can be isolated from HIV\u2010infected patients with NHL and without NHL. This findings point to the need for further molecular characterization and functional studies of SIBCs, which may prove the value of SIBCs in the diagnosis, prognoses, and screening for NHL among HIV\u2010infected patients.<\/jats:p>","DOI":"10.1002\/cam4.2508","type":"journal-article","created":{"date-parts":[[2019,9,20]],"date-time":"2019-09-20T09:44:22Z","timestamp":1568972662000},"page":"6741-6755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Isolation and characterization of spontaneously immortalized B\u2010lymphocyte lines from HIV\u2010infected patients with and without non\u2010Hodgkin's Lymphoma"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3471-969X","authenticated-orcid":false,"given":"Ke","family":"Zhuang","sequence":"first","affiliation":[{"name":"ABSL\u2010III Laboratory at the Center for Animal Experiment State Key Laboratory of Virology Wuhan University 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