{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:28:36Z","timestamp":1788870516019,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,5,3]],"date-time":"2014-05-03T00:00:00Z","timestamp":1399075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council Taiwan","doi-asserted-by":"publisher","award":["NSC98-3114-P-016-001-Y"],"award-info":[{"award-number":["NSC98-3114-P-016-001-Y"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council Taiwan","doi-asserted-by":"publisher","award":["NSC99-3113-P-016-001"],"award-info":[{"award-number":["NSC99-3113-P-016-001"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council Taiwan","doi-asserted-by":"publisher","award":["NSC 102-2628-B-038 -010 -MY3"],"award-info":[{"award-number":["NSC 102-2628-B-038 -010 -MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2014,8]]},"abstract":"Abstract<\/jats:title>\n \n We aimed to determine whether\n \n PAX<\/jats:styled-content>\n 1\/\n SOX<\/jats:styled-content>\n 1\n <\/jats:italic>\n methylation could be translated to clinical practice for cervical neoplasia detection when used alone and in combination with current cytology\u2010based Pap screening. We conducted a multicenter case\u2013control study in 11 medical centers in Taiwan from December 2009 to November 2010. Six hundred seventy\u2010six patients were included in the analysis, including 330 in the training set and 346 in the testing set. Multiplex quantitative methylation\u2010specific polymerase chain reaction (\n PCR<\/jats:styled-content>\n ) was performed with a TaqMan probe system using a LightCycler 480 Real\u2010Time\n PCR<\/jats:styled-content>\n System (Roche). The level of human papilloma virus (\n HPV<\/jats:styled-content>\n ) was analyzed using a Hybrid Capture 2 system (Digene). Receiver operating characteristic curves were generated to obtain the best cutoff values from the training data set. The sensitivities, specificities, and accuracies were validated in the testing set. The sensitivities for methylated (\n \n m<\/jats:italic>\n <\/jats:sup>\n )\n \n PAX<\/jats:styled-content>\n 1\n <\/jats:italic>\n \n m<\/jats:italic>\n <\/jats:sup>\n and\n \n SOX<\/jats:styled-content>\n 1\n <\/jats:italic>\n \n m<\/jats:italic>\n <\/jats:sup>\n and\n HPV<\/jats:styled-content>\n testing for detecting\n CIN<\/jats:styled-content>\n 3\n +<\/jats:sup>\n lesions were 0.64, 0.71, and 0.89, and the specificities were 0.91, 0.77, and 0.68, respectively. Combined parallel testing of\n \n PAX<\/jats:styled-content>\n 1\n <\/jats:italic>\n \n m<\/jats:italic>\n <\/jats:sup>\n \/\n \n SOX<\/jats:styled-content>\n 1\n <\/jats:italic>\n \n m<\/jats:italic>\n <\/jats:sup>\n tests with Pap smearing showed superior specificity (0.84\/0.71 vs. 0.66, respectively) and similar sensitivity (0.93\/0.96 vs. 0.97) to the combination of Pap smear results and\n HPV<\/jats:styled-content>\n testing. Thus, combined parallel testing using Pap smears and\n \n PAX<\/jats:styled-content>\n 1\n <\/jats:italic>\n or\n \n SOX<\/jats:styled-content>\n 1\n <\/jats:italic>\n methylation tests may provide better performance than a combination of Pap smears with\n HPV<\/jats:styled-content>\n testing in detection for cervical neoplasia.\n <\/jats:p>","DOI":"10.1002\/cam4.253","type":"journal-article","created":{"date-parts":[[2014,5,3]],"date-time":"2014-05-03T05:50:41Z","timestamp":1399096241000},"page":"1062-1074","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["\n PAX<\/scp>\n 1\n <\/i>\n \/\n \n SOX<\/scp>\n 1\n <\/i>\n \u00a0\n DNA<\/scp>\n methylation and cervical neoplasia detection: a Taiwanese Gynecologic Oncology Group (\n TGOG<\/scp>\n ) study"],"prefix":"10.1002","volume":"3","author":[{"given":"Hung\u2010Cheng","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Shuang Ho Hospital Taipei Medical University Taipei Taiwan"},{"name":"Department of Obstetrics and Gynecology Tri\u2010Service General Hospital Taipei Taiwan"},{"name":"Department of Obstetrics and Gynecology Taipei Medical University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Che","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kaohsiung Chang Gung Memorial Hospital Kaohsiung Taiwan"},{"name":"Chang Gung University College of Medicine Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tze\u2010Chien","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Mackay Memorial Hospital Taipei Taiwan"},{"name":"General Education Center Tatung University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huei\u2010Jean","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Chang Gung Memorial Hospital Taoyuan Taiwan"},{"name":"Chang Gung University College of Medicine Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Min","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology National Cheng Kung University Hospital Tainan Taiwan"},{"name":"College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Hau","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology National Taiwan University Taipei Taiwan"},{"name":"College of Medicine and National Taiwan University Hospital Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tang\u2010Yuan","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Buddhist Tzu Chi General Hospital Tzu Chi University Hualien Taiwan"},{"name":"Graduate Institute of Medical Science Tzu Chi University Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih\u2010Tien","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Taichung Veterans General Hospital Taichung Taiwan"},{"name":"College of Medicine China Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kaohsiung Veterans General hospital Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao\u2010Ching","family":"Hung","sequence":"additional","affiliation":[{"name":"College of Medicine China Medical University Taichung Taiwan"},{"name":"Department of Obstetrics and Gynecology China Medical University Hospital Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo\u2010Chang","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Taipei Veterans General Hospital Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu\u2010Hsien","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Tri\u2010Service General Hospital Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kung\u2010Liahng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Taipei Medical University Taipei 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