{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T22:02:53Z","timestamp":1771020173217,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T00:00:00Z","timestamp":1577750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004572","name":"National Health and Family Planning Commission of the People's Republic of China","doi-asserted-by":"crossref","award":["W2014RQ29"],"award-info":[{"award-number":["W2014RQ29"]}],"id":[{"id":"10.13039\/501100004572","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We aimed to explore the molecular substrate underlying EGFR\u2010TKI resistance and investigate the effects of N\u2010acetylcysteine (NAC) on reversing EGFR\u2010TKI resistance. In the current research, the effects of NAC in combination with gefitinib on reversing gefitinib resistance were examined using CCK\u20108 assay, combination index (CI) method, matrigel invasion assay, wound\u2010healing assay, flow cytometry, western blot, and quantitative real\u2010time PCR (qRT\u2010PCR). CCK8 assay showed that NAC plus gefitinib combination overcame EGFR\u2010TKI resistance in non\u2010small cell lung cancer (NSCLC) cells by lowering the value of half maximal inhibitory concentration (IC50). CI calculations demonstrated a synergistic effect between the two drugs (CI\u00a0&lt;\u00a01). Matrigel invasion\u00a0assay and wound healing assay demonstrated a decrease in migration and invasion ability of PC\u20109\/GR cells after NAC and gefitinib treatment. Flow cytometry displayed enhanced apoptosis in the combination group. Western blot and qRT\u2010PCR revealed that increased E\u2010cadherin and decreased vimentin in the combination group. When PP2 was administered with gefitinib, the same effects were seen. Our findings suggest that NAC could restore the sensitivity of gefitinib\u2010resistant NSCLC cells to gefitinib via suppressing Src activation and reversing epithelial\u2010mesenchymal transition.<\/jats:p>","DOI":"10.1002\/cam4.2610","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T12:39:50Z","timestamp":1577795990000},"page":"1495-1502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Combined treatment with N\u2010acetylcysteine and gefitinib overcomes drug resistance to gefitinib in NSCLC cell line"],"prefix":"10.1002","volume":"9","author":[{"given":"Jun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Chongqing Medical University Chongqing 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