{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T02:21:52Z","timestamp":1782094912299,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T00:00:00Z","timestamp":1574208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30\u2010CA76292"],"award-info":[{"award-number":["P30\u2010CA76292"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R21CA194932"],"award-info":[{"award-number":["R21CA194932"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008792","name":"Novartis Pharma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>For the advancement of cancer research, the collection of tissue specimens from drug\u2010resistant tumors after targeted therapy is crucial. Although patients with lung cancer are often provided targeted therapy, post\u2010therapy specimens are not routinely collected due to the risks of collection, limiting the study of targeted therapy resistance mechanisms. Posthumous rapid tissue donation (RTD) is an expedient collection process that provides an opportunity to understand treatment\u2010resistant lung cancers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Consent to participate in the thoracic RTD protocol was obtained during patient care. When death occurred, tumor and paired non\u2010tumor, cytology, and blood specimens were collected within 48\u00a0hours and preserved as formalin\u2010fixed and frozen specimens. Tissue sections were evaluated with hematoxylin and eosin staining and immunohistochemistry (IHC) against multiple biomarkers, including various programmed death ligand 1 (PD\u2010L1) clones. Next\u2010generation sequencing was performed on 13 specimens from 5 patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Postmortem specimens (N\u00a0=\u00a0180) were well preserved from 9 patients with lung cancer. PD\u2010L1 IHC revealed heterogeneity within and between tumors. An <jats:italic>AGK\u2010BRAF<\/jats:italic> fusion was newly identified in tumor from a donor with a known echinoderm microtubule\u2010associated protein\u2010like 4 to anaplastic lymphoma kinase (<jats:italic>EML4\u2010ALK<\/jats:italic>) fusion and history of anaplastic lymphoma kinase (ALK) inhibitor therapy. RNA expression analysis revealed a clonal genetic origin of metastatic cancer cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Post\u2010therapy specimens demonstrated PD\u2010L1 heterogeneity and an acyl glycerol kinase to B\u2010rapidly accelerated fibrosarcoma (<jats:italic>AGK\u2010BRAF<\/jats:italic>) fusion in a patient with an <jats:italic>EML4\u2010ALK<\/jats:italic>\u2013positive lung adenocarcinoma\u00a0as a potential resistance mechanism to ALK inhibitor therapy. Rapid tissue donation collection of postmortem tissue from lung cancer patients is a novel approach to cancer research that enables studies of molecular evolution and drug resistance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.2670","type":"journal-article","created":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T16:43:03Z","timestamp":1574268183000},"page":"225-237","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A community\u2010based lung cancer rapid tissue donation protocol provides high\u2010quality drug\u2010resistant specimens for proteogenomic analyses"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5950-3970","authenticated-orcid":false,"given":"Theresa A.","family":"Boyle","sequence":"first","affiliation":[{"name":"Department of Oncologic Science Morsani College of Medicine University of South Florida Tampa FL USA"},{"name":"Anatomic Pathology Department H. 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