{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T10:44:39Z","timestamp":1783075479244,"version":"3.54.6"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672400"],"award-info":[{"award-number":["81672400"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672788"],"award-info":[{"award-number":["81672788"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Circulating microRNAs (miRNAs) have become reliable sources of non\u2010invasive biomarkers for cancer diagnosis. Identification of promising miRNA biomarkers in plasma might benefit a lot to the detection of nasopharyngeal carcinoma (NPC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The Exiqon miRNA qPCR panel was used in the screening stage to identify candidate miRNAs, which were further verified by quantitative reverse transcription polymerase chain reaction (qRT\u2010PCR) in the following three stages among plasma samples from 200 NPC patients and 189 healthy donors (as normal controls [NCs]). The identified miRNAs were further explored in tissue specimens (48 NPC vs 32 NCs) and plasma exosomes (32 NPC vs 32 NCs). Survival analyses were ultimately conducted by Cox regression models and Kaplan\u2010Meier curves using log\u2010rank tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified a 7\u2010miRNA signature including let\u20107b\u20105p, miR\u2010140\u20103p, miR\u2010144\u20103p, miR\u201017\u20105p, miR\u201020a\u20105p, miR\u201020b\u20105p, and miR\u2010205\u20105p in plasma for NPC diagnosis after four\u2010stage validation. The areas under the receiver operating characteristic curve (AUCs) for the signature were 0.879, 0.884, 0.921, and 0.807 for the training, testing, external validation stage, and the combined three stages, respectively. In NPC tissues, miR\u2010144\u20103p, miR\u201017\u20105p, miR\u201020a\u20105p, and miR\u2010205\u20105p were consistently up\u2010regulated while let\u20107b\u20105p and miR\u2010140\u20103p were significantly down\u2010regulated compared to NCs. However, none of the seven identified miRNAs were dysregulated in plasma\u2010derived exosomes in NPC patients. As to survival analysis, none of the seven miRNAs seemed to be associated with NPC prognosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We identified a 7\u2010miRNA signature in plasma as promising non\u2010invasive biomarkers for NPC detection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.2676","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T18:11:45Z","timestamp":1576779105000},"page":"1230-1241","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Identification of a 7\u2010microRNA signature in plasma as promising biomarker for nasopharyngeal carcinoma detection"],"prefix":"10.1002","volume":"9","author":[{"given":"Huo","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Oncology First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu PR China"},{"name":"Department of Medical Oncology Northern Jiangsu 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