{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T07:56:00Z","timestamp":1764402960706,"version":"3.44.0"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T00:00:00Z","timestamp":1574640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011305","name":"Comprehensive Cancer Center, University of Alabama at Birmingham","doi-asserted-by":"publisher","award":["P30CA013148"],"award-info":[{"award-number":["P30CA013148"]}],"id":[{"id":"10.13039\/100011305","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>There are no HPV\u2010based measures for managing anal cancer (AC) in HIV\u2010infected (HIV+) men who have sex with men (MSM) because of the high positivity of high\u2010risk (HR)\u2010HPVs. As next\u2010generation sequencing (NGS) is able to describe the composition of HPVs as percent (%) reads rather than positive vs negative results, we used NGS approach to detect HPVs in anal samples of HIV+\u00a0MSM to test its ability to differentiate those who are diagnosed with atypical squamous cells of unknown significance or greater (ASCUS+) from those who are free of such lesions and to understand the burden of HPV infections in relation to HPV vaccines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Study included 81 HIV+\u00a0MSM characterized for demographics, patient\u2010reported outcome measures, HIV related laboratory measures and anal cytology. We summarized NGS HPV data using % read cut points (&gt;0%\u2010&gt;30%) and tested the relationship between % reads of HR\u2010HPVs and risk of ASCUS+\u00a0using logistic regression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty\u2010six HPVs were detected at the &gt;0% read cut point. The prevalence of any HR\u2010HPVs varied from 100% to 40% with &gt;0% to &gt;30% reads\u00a0while \u226599% were infected with HR\u2010HPVs included or not included in the 9 valent HPV vaccine at the &gt;0% read cut point. MSM with &gt;30% HR\u2010HPV reads were 4.5 times more likely to be diagnosed with ASCUS+\u00a0compared to\u00a0\u226430% reads (<jats:italic>P\u00a0<\/jats:italic>=\u00a0.033).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>NGS\u2010based approach is more accurate than PCR\u2010based HPV testing for identifying HIV+\u00a0MSM at risk for developing AC. We raise the concern regarding the efficacy of current HPV vaccines for preventing AC in this high\u2010risk population.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.2720","type":"journal-article","created":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T20:37:08Z","timestamp":1574714228000},"page":"807-815","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A rigorous exploration of anal HPV genotypes using a next\u2010generation sequencing (NGS) approach in HIV\u2010infected men who have sex with men at risk for developing anal cancer"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3857-7035","authenticated-orcid":false,"given":"Chandrika J.","family":"Piyathilake","sequence":"first","affiliation":[{"name":"Department of Nutrition Sciences The University of Alabama at Birmingham (UAB) Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suguna","family":"Badiga","sequence":"additional","affiliation":[{"name":"Department of Nutrition Sciences The University of Alabama at Birmingham (UAB) Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranjit","family":"Kumar","sequence":"additional","affiliation":[{"name":"UAB Center for Clinical &amp; Translational Science The University of Alabama at Birmingham (UAB) Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Crowley","sequence":"additional","affiliation":[{"name":"Department of Genetics The University of Alabama at Birmingham (UAB) Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greer A.","family":"Burkholder","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine The University of Alabama at Birmingham (UAB) Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L.","family":"Raper","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine The University of Alabama at Birmingham (UAB) Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081355"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis613"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30716"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3018(17)30125-X"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e3283023f78"},{"key":"e_1_2_8_7_1","unstructured":"FerlayJ SoerjomataramI ErvikM et al. eds.Globocan 2012: estimated cancer incidence mortality and prevalence worldwide in 2012. 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