{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T19:39:57Z","timestamp":1740166797868,"version":"3.37.3"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,8]],"date-time":"2019-12-08T00:00:00Z","timestamp":1575763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009601","name":"Beijing Municipal Administration of Hospitals\u2019 Ascent Plan","doi-asserted-by":"crossref","award":["DFL20180701"],"award-info":[{"award-number":["DFL20180701"]}],"id":[{"id":"10.13039\/501100009601","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Special Fund for the Capital Characteristic Clinical Medicine Development Project","award":["Z161100000516077"],"award-info":[{"award-number":["Z161100000516077"]}]},{"name":"Beijing Municipal Grant for Medical Talents Group on Peritoneal Surface Oncology","award":["2017400003235J007"],"award-info":[{"award-number":["2017400003235J007"]}]},{"name":"Health Science Promotion Project of Beijing; Beijing Natural Science Foundation","award":["7172108"],"award-info":[{"award-number":["7172108"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pseudomyxoma peritonei (PMP) is an indolent malignancy and insensitive to systemic chemotherapy. The authors established patient\u2010derived xenograft (PDX) model of PMP, and evaluated the efficacy and toxicity of intraperitoneal (i.p.) administration of 5\u2010fluorouracil (5\u2010FU) in this model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human PMP sample was collected to establish subcutaneous (s.c.) and i.p. model. In vivo study of i.p. injection of 5\u2010FU was performed in i.p. model, with experimental peritoneal cancer index (ePCI) score and pathological examinations for evaluating the efficacy and toxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both s.c. and i.p. models were constructed. The average passage interval of s.c. model was 44.2\u00a0\u00b1\u00a05.2\u00a0days, and the i.p. model was characterized by disseminated solid tumor nodules in abdominal\u2010pelvic cavity. Both models were diagnosed as peritoneal mucinous carcinomatosis with signet ring cells (PMCA\u2010S). Immunohistochemical characteristics was similar to human. <jats:italic>GNAS<\/jats:italic> mutation was detected in both model and patient. In the in vivo study, average ePCI of treatment group was lower than control and vehicle group (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.004). Histopathology revealed obvious tumor necrosis in treatment group, and decreased Ki67 positive rate (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.010). In toxicity study, 5\u2010FU significantly influenced body weight (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.010) and 1 animal from treatment group died on day 14. Congestive splenomegaly was observed (88.9%). Hepatotoxicity presented as acidophilic body (55.6%), cholestasis (100%), bile canaliculus hyperplasia and obstruction (22.2%), and lymphocyte accumulation (77.8%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PDX model of PMCA\u2010S was established successfully, and i.p. 5\u2010FU could inhibit tumor proliferation and progression, with decreased Ki67 positive rate and ePCI score. Hepatotoxicity was the main side effect.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.2766","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T07:41:16Z","timestamp":1575877276000},"page":"1104-1114","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Establishment of patient\u2010derived xenograft model of peritoneal mucinous carcinomatosis with signet ring cells and in vivo study on the efficacy and toxicity of intraperitoneal injection of 5\u2010fluorouracil"],"prefix":"10.1002","volume":"9","author":[{"given":"Yu\u2010Lin","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Peritoneal Cancer Surgery Beijing Shijitan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Peritoneal Cancer Surgery Beijing Shijitan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng\u2010Cai","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pathology Beijing Shijitan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pathology Beijing Shijitan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ru","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Peritoneal Cancer Surgery Beijing Shijitan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Ran","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Peritoneal Cancer Surgery Beijing Shijitan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Bin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Myxoma Aerospace Central Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of General Surgery Chinese PLA General Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Chen","sequence":"additional","affiliation":[{"name":"Thorgene Co., Ltd. Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6018-6538","authenticated-orcid":false,"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Peritoneal Cancer Surgery Beijing Shijitan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000535"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/02656736.2017.1310938"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2007.04.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1067\/j.cpsurg.2008.04.003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2168.1998.00882.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.39.7166"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.21702"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1980.01380040037006"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10434-999-0727-7"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-016-5529-0"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.26596"},{"key":"e_1_2_8_13_1","first-page":"390","article-title":"Recombinant interleukin\u20102 and adoptively transferred lymphokine\u2010activated killer cells in the treatment of experimental peritoneal carcinomatosis","volume":"36","author":"Steller E","year":"1985","journal-title":"Surg Forum"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.10.009"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2514"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts271"},{"issue":"10","key":"e_1_2_8_17_1","first-page":"4051","article-title":"In vivo model of pseudomyxoma peritonei for novel candidate drug discovery","volume":"29","author":"Chua TC","year":"2009","journal-title":"Anticancer Res"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.03.004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-7-116"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2613.2010.00721.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.640"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/gm559"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2013.132"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.47"},{"issue":"9","key":"e_1_2_8_25_1","first-page":"530","article-title":"Taurine as a protective agent for 5\u2010fluorouracil\u2010induced hepatic damage related to oxidative stress","volume":"71","author":"Fukuno S","year":"2016","journal-title":"Pharmazie"},{"issue":"18","key":"e_1_2_8_26_1","first-page":"5790","article-title":"Early postoperative intraperitoneal chemotherapy as an adjuvant therapy to surgery for peritoneal carcinomatosis from gastrointestinal cancer: pharmacological studies","volume":"50","author":"Sugarbaker PH","year":"1990","journal-title":"Cancer Res"},{"issue":"4","key":"e_1_2_8_27_1","first-page":"404","article-title":"The protective effects of naringin against 5\u2010fluorouracil\u2010induced hepatotoxicity and nephrotoxicity in rats","volume":"21","author":"Gelen V","year":"2018","journal-title":"Iran J Basic Med Sci"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3328"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000018682"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19900401)65:7<1495::AID-CNCR2820650708>3.0.CO;2-1"},{"issue":"3","key":"e_1_2_8_31_1","first-page":"213","article-title":"Pseudomyxoma peritonei: treatment with intraperitoneal 5\u2010fluorouracil","volume":"14","author":"Nasr MF","year":"1993","journal-title":"Eur J Gynaecol Oncol"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1998.225"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2004.08.033"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001813-199803000-00012"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4804(88)90045-5"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcam4.2766","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.2766","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.2766","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.2766","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T10:47:51Z","timestamp":1723632471000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.2766"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,8]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/cam4.2766"],"URL":"https:\/\/doi.org\/10.1002\/cam4.2766","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"type":"print","value":"2045-7634"},{"type":"electronic","value":"2045-7634"}],"subject":[],"published":{"date-parts":[[2019,12,8]]},"assertion":[{"value":"2019-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}