{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T12:03:51Z","timestamp":1786622631771,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MERMAID-2. Open Access Publishing Fund of University\u00a0of Tuebingen"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Several studies have demonstrated that the viral genome can be methylated by the host cell during progression from persistent infection to cervical cancer. The aim of this study was to investigate whether methylation at a specific site could predict the development of viral persistence and whether viral load shows a correlation with specific methylation patterns. HPV16\u2010positive samples from women aged 20\u201329\u00a0years (n\u00a0=\u00a099) with a follow\u2010up time of 13\u00a0years, were included from a Danish cohort comprising 11\u00a0088 women. Viral load was measured by real\u2010time PCR and methylation status was determined for 39 CpG sites in the upstream regulatory region (URR), <jats:italic>E6\/E7,<\/jats:italic> and <jats:italic>L1<\/jats:italic> region of HPV16 by next\u2010generation sequencing. Participants were divided into two groups according to whether they were persistently (\u2265\u00a024\u00a0months) or transiently HPV16 infected. The general methylation status was significantly different between women with a persistent and women with a transient infection outcome (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.025). One site located in <jats:italic>L1<\/jats:italic> (nt.\u00a05962) was statistically significantly (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.00048) different in the methylation status after correction using the Holm\u2010Sidak method (alpha\u00a0=\u00a00.05). Correlation analyses of samples from HPV16 persistently infected women suggest that methylation is higher although viral load is lower. This study indicates that methylation at position 5962 of the HPV16 genome within the <jats:italic>L1<\/jats:italic> gene might be a predictive marker for the development of a persistent HPV16 infection.<\/jats:p>","DOI":"10.1002\/cam4.2771","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:51:30Z","timestamp":1576785090000},"page":"1058-1068","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Methylation of CpG 5962 in L1 of the human papillomavirus 16 genome as a potential predictive marker for viral persistence: A prospective large cohort study using cervical swab samples"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5424-3807","authenticated-orcid":false,"given":"Jasmin","family":"Fertey","sequence":"first","affiliation":[{"name":"Medical Virology Institute of Medical Virology University Hospital of Tuebingen Tuebingen 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