{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T09:16:32Z","timestamp":1770455792055,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T00:00:00Z","timestamp":1580860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatocellular carcinoma (HCC) is one of the most deadly cancer worldwide. Multiple long noncoding RNAs (lncRNAs) are recently identified as crucial oncogenic factors or tumor suppressors. In this study, we explored the functon and mechanism of lncRNA double homeobox A pseudogene 8 (DUXAP8) in the progression of HCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Expression levels of DUXAP8 in HCC tissue samples were measured using qRT\u2010PCR. The association between pathological indexes and the expression of DUXAP8 was also analyzed. Human HCC cell lines SMMC\u20107721 and QSG\u20107701 were used in in vitro studies. CCK\u20108 assay was used to assess the effect of DUXAP8 on HCC cell line proliferation. Scratch healing assay and Transwell assay were conducted to detect the effect of DUXAP8 on migration and invasion. Furthermore, dual\u2010luciferase reporter assay was used to confirm targeting relationship between miR\u2010422a and DUXAP8. Additionally, Western blot was used to detect the regulatory function of DUXAP8 on pyruvate dehydrogenase kinase 2 (PDK2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DUXAP8 expression HCC clinical samples was significantly increased and this was correlated with unfavorable pathological indexes. High expression of DUXAP8 was associated with shorter overall survival time of patients. Its overexpression remarkably facilitated the proliferation, metastasis, and epithelial\u2010mesenchymal transition of HCC cells. Accordingly, knockdown of it suppressed the malignant phenotypes of HCC cells. Overexpression of DUXAP8 significantly reduced the expression of miR\u2010422a by sponging it, but enhanced the expression of PDK2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>DUXAP8 was a sponge of tumor suppressor miR\u2010422a in HCC, enhanced the expression of PDK2 indirectly, and functioned as an oncogenic lncRNA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.2861","type":"journal-article","created":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T12:41:31Z","timestamp":1580906491000},"page":"2480-2490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Long noncoding RNA DUXAP8 contributes to the progression of hepatocellular carcinoma via regulating miR\u2010422a\/PDK2 axis"],"prefix":"10.1002","volume":"9","author":[{"given":"Feifei","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Oncology Fifth Clinical Medical College Guilin Medical University Guilin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Oncology Fifth Clinical Medical College Guilin Medical University Guilin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0517-3042","authenticated-orcid":false,"given":"Dandan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Oncology Jining NO.1 People's Hospital; Affiliated Jining NO.1 People's Hospital Jining Medical University Jining People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Oncology Fifth Clinical Medical College Guilin Medical University Guilin People's Republic of 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