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The clinical features and splicing events of patients with HCC were downloaded from The Cancer Genome Atlas (TCGA). Differentially expressed AS (DEAS) were compared between HCC and adjacent normal samples. Univariate Cox regression analysis was used to determine changes in DEAS associated with overall survival (OS). A PI was generated from OS\u2010associated DEASs using Kaplan\u2010Meier curves, receiver operating characteristic (ROC) curves, multivariate Cox regression, and cluster analysis. Then, the correlation between DEASs and splicing factors was assessed, followed by functional and pathway enrichment analysis. We identified 34\u00a0163 ASs of 8985 genes in HCC, and 153 OS\u2010ASs were identified using univariate Cox regression analysis. Low\u2010 and high\u2010PI groups were determined based on the median \u201cPI\u2010ALL\u201d value according to significantly different survival (<jats:italic>P<\/jats:italic>\u00a0=\u00a02.2e\u00a0\u2212\u00a016). The ROC curve of all PI (PI\u2010ALL) had an area under the curve (AUC) of 0.993 for survival status in patients with HCC. A potential regulatory network associated with prognosis of patients with HCC was established. Enrichment analysis also resulted in the identification of several pathways potentially associated with carcinogenesis and progression of HCC. Four clusters were identified that were associated with clinical features and prognosis. Our study generated comprehensive profiles of ASs in HCC. The interaction network and functional connections were used to elucidate the underlying mechanisms of AS in HCC.<\/jats:p>","DOI":"10.1002\/cam4.2875","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T08:31:11Z","timestamp":1579854671000},"page":"2171-2180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Profiles of prognostic alternative splicing signature in hepatocellular carcinoma"],"prefix":"10.1002","volume":"9","author":[{"given":"Fangming","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Comprehensive Intervention Henan Provincial People's Hospital Zhengzhou China"},{"name":"Department of Comprehensive Intervention Zhengzhou University People's Hospital Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qifeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging and Interventional Radiology Sun Yat\u2010sen University Cancer Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaojun","family":"Liu","sequence":"additional","affiliation":[{"name":"Biotherapy Center the First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoran","family":"Duan","sequence":"additional","affiliation":[{"name":"Biotherapy Center the First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Oncology Henan Provincial People's Hospital Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Intervention Henan Provincial People's Hospital Zhengzhou China"},{"name":"Department of Comprehensive Intervention Zhengzhou University People's Hospital Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huicun","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Intervention Henan Provincial People's Hospital Zhengzhou China"},{"name":"Department of Comprehensive Intervention Zhengzhou University People's Hospital Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging and Interventional Radiology Sun Yat\u2010sen University Cancer Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2314-3114","authenticated-orcid":false,"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Intervention Henan Provincial People's Hospital Zhengzhou China"},{"name":"Department of 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