{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T12:34:02Z","timestamp":1781008442490,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T00:00:00Z","timestamp":1580688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871940"],"award-info":[{"award-number":["81871940"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902417"],"award-info":[{"award-number":["81902417"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Kras mutation and abnormal immune status are associated with pancreatic cancer development and progression. In this study, we evaluated the Kras mutation status in circulating tumor DNA and circulating T cell subsets in a cohort of advanced pancreatic cancer patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Samples were retrospectively obtained from a series of 210 pathological advanced pancreatic cancer patients between 2012 and 2014. The Kras mutation status was detected in cell\u2010free circulating tumor DNA (ctDNA) by ddPCR and circulating T cell subsets were analyzed by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Univariate analysis found that tumor node metastasis (TNM) stage, chemotherapy, circulating regulatory T cells, CA19\u20109 levels, CA125 levels, and Kras<jats:sup>G12D<\/jats:sup> and Kras<jats:sup>G12V<\/jats:sup> mutations were significantly related to overall survival in advanced pancreatic cancer patients. Multivariate analysis identified that TNM stage (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.03, HR:1.422), Tregs (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.004, HR:1.522), CA19\u20109 levels (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.009, HR:1.488), Kras<jats:sup>G12D<\/jats:sup> mutation (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.044, HR:1.353), and Kras<jats:sup>G12V<\/jats:sup> mutation (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.001, HR:1.667) were independent prognostic markers. Furthermore, we found that Kras<jats:sup>G12V<\/jats:sup> mutation in ctDNA was correlated with high circulating proportion of Tregs, and patients with both Kras<jats:sup>G12V<\/jats:sup> mutation and high levels of Tregs were associated with extremely poor survival in advanced pancreatic cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Kras<jats:sup>G12V<\/jats:sup> mutation was associated with high circulating regulatory T cell levels, and both of them predicted worse prognosis in advanced pancreatic cancer patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.2895","type":"journal-article","created":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T07:53:45Z","timestamp":1580802825000},"page":"2153-2159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Kras mutation correlating with circulating regulatory T cells predicts the prognosis of advanced pancreatic cancer patients"],"prefix":"10.1002","volume":"9","author":[{"given":"He","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Pancreatic Surgery Fudan University Shanghai Cancer Center Shanghai China"},{"name":"Department of Oncology Shanghai Medical College Fudan University Shanghai China"},{"name":"Shanghai Pancreatic Cancer Institute Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guopei","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pancreatic Surgery Fudan University Shanghai Cancer Center Shanghai China"},{"name":"Department of Oncology Shanghai Medical College Fudan University Shanghai China"},{"name":"Shanghai Pancreatic Cancer Institute Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaizhou","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pancreatic Surgery Fudan University Shanghai Cancer Center Shanghai China"},{"name":"Department of 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