{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:34Z","timestamp":1772064034186,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T00:00:00Z","timestamp":1585785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Intraductal papillary mucinous neoplasm (IPMN) is an intraepithelial precancerous lesion of pancreatic ductal adenocarcinoma (PDAC) that progresses from adenoma to carcinoma, and long noncoding RNAs (lncRNA) might be involved in the tumorigenesis. In this study, we obtained the expression profiles of more than 4000 lncRNAs by probe reannotation of a microarray dataset. As a correlation network\u2010based systems biology method, weighted gene coexpression network analysis (WGCNA) was used to find clusters of highly correlated lncRNAs in the tumorigenesis of IPMN, which covered four stepwise stages from normal main pancreatic duct to invasive IPMN. In the most relevant module (R<jats:sup>2<\/jats:sup>\u00a0=\u00a0\u22120.75 and <jats:italic>P<\/jats:italic>\u00a0=\u00a05E\u201005), three hub lncRNAs were identified (HAND2\u2010AS1, CTD\u20102033D15.2, and lncRNA\u2010TFG). HAND2\u2010AS1 and CTD\u20102033D15.2 were negatively correlated with the tumorigenesis (<jats:italic>P<\/jats:italic> in one\u2010way ANOVA test\u00a0=\u00a01.45E\u201007 and 1.39E\u20100.5), while lncRNA\u2010TFG were positively correlated with the tumorigenesis (<jats:italic>P<\/jats:italic>\u00a0=\u00a03.99E\u201008). The validation set reached consistent results (<jats:italic>P<\/jats:italic>\u00a0=\u00a02.66E\u201003 in HAND2\u2010AS1, 1.47E\u201004 in CTD\u20102033D15.2 and 6.23E\u201008 in lncRNA\u2010TFG). In functional enrichment analysis, the target genes of microRNAs targeting also these lncRNAs were overlapped in multiple biological processes, pathways and malignant diseases including pancreatic cancer. In survival analysis, patients with higher expression of HAND2\u2010AS1\u2010targeted and CTD\u20102033D15.2\u2010targeted microRNAs showed a significantly poorer prognosis in PDAC, while high expression of lncRNA\u2010TFG\u2010targeted microRNAs demonstrated an obviously better prognosis (log\u2010rank <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). In conclusion, by coexpression network analysis of the lncRNA profiles, three key lncRNAs were identified in association with the tumorigenesis of IPMN, and those lncRNAs might act as early diagnostic biomarkers or therapeutic targets in pancreatic cancer.<\/jats:p>","DOI":"10.1002\/cam4.2927","type":"journal-article","created":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T15:16:59Z","timestamp":1585840619000},"page":"3840-3851","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Identification of key lncRNAs in the tumorigenesis of intraductal pancreatic mucinous neoplasm by coexpression network analysis"],"prefix":"10.1002","volume":"9","author":[{"given":"Jun","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery The Central Hospital of Enshi Autonomous Prefecture Enshi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Central Hospital of Enshi Autonomous Prefecture Enshi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Central Hospital of Enshi Autonomous Prefecture Enshi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Central Hospital of Enshi Autonomous Prefecture Enshi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinghe","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Central Hospital of Enshi Autonomous Prefecture Enshi 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